In vivo fluorescent optical imaging of cytotoxic T lymphocyte migration using IRDye800CW near-infrared dye

Aaron E. Foster; Sunkuk Kwon; Shi Ke; An Lu; Karen Eldin; Eva Sevick-Muraca; Cliona M. Rooney

doi:10.1364/AO.47.005944

In vivo fluorescent optical imaging of cytotoxic T lymphocyte migration using IRDye800CW near-infrared dye

Aaron E. Foster, Sunkuk Kwon, Shi Ke, An Lu, Karen Eldin, Eva Sevick-Muraca, Cliona M. Rooney

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

We describe a method to measure in vivo migration of human T cells by using the near-infrared (NIR) dye IRDye800CW, Labeling of Epstein-Barr virus-specific T cells with IRDye800CW did not affect viability, proliferation, or T cell function. Following tail vein injection into mice bearing subcutaneous tumors, the NIR signal could be measured in vivo at the tumor site. Analysis of tumors revealed T cell infiltration and an increased NIR signal, confirming T cell migration. To test specific migration with IRDye800CW, tumors were modified to express CCL5 to measure site-specific migration. The NIR signal was increased at CCL5-secreting tumors compared with control tumors. Together, these data suggest that IRDye800CW may be used to study the trafficking of T cells in a small animal model and may have potential as a shortterm reporter molecule for human immunotherapy studies.

Original language	English (US)
Pages (from-to)	5944-5952
Number of pages	9
Journal	Applied Optics
Volume	47
Issue number	31
DOIs	https://doi.org/10.1364/AO.47.005944
State	Published - Nov 1 2008

ASJC Scopus subject areas

Atomic and Molecular Physics, and Optics
Engineering (miscellaneous)
Electrical and Electronic Engineering

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Cite this

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title = "In vivo fluorescent optical imaging of cytotoxic T lymphocyte migration using IRDye800CW near-infrared dye",

abstract = "We describe a method to measure in vivo migration of human T cells by using the near-infrared (NIR) dye IRDye800CW, Labeling of Epstein-Barr virus-specific T cells with IRDye800CW did not affect viability, proliferation, or T cell function. Following tail vein injection into mice bearing subcutaneous tumors, the NIR signal could be measured in vivo at the tumor site. Analysis of tumors revealed T cell infiltration and an increased NIR signal, confirming T cell migration. To test specific migration with IRDye800CW, tumors were modified to express CCL5 to measure site-specific migration. The NIR signal was increased at CCL5-secreting tumors compared with control tumors. Together, these data suggest that IRDye800CW may be used to study the trafficking of T cells in a small animal model and may have potential as a shortterm reporter molecule for human immunotherapy studies.",

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T1 - In vivo fluorescent optical imaging of cytotoxic T lymphocyte migration using IRDye800CW near-infrared dye

AU - Foster, Aaron E.

AU - Kwon, Sunkuk

AU - Ke, Shi

AU - Lu, An

AU - Eldin, Karen

AU - Sevick-Muraca, Eva

AU - Rooney, Cliona M.

PY - 2008/11/1

Y1 - 2008/11/1

N2 - We describe a method to measure in vivo migration of human T cells by using the near-infrared (NIR) dye IRDye800CW, Labeling of Epstein-Barr virus-specific T cells with IRDye800CW did not affect viability, proliferation, or T cell function. Following tail vein injection into mice bearing subcutaneous tumors, the NIR signal could be measured in vivo at the tumor site. Analysis of tumors revealed T cell infiltration and an increased NIR signal, confirming T cell migration. To test specific migration with IRDye800CW, tumors were modified to express CCL5 to measure site-specific migration. The NIR signal was increased at CCL5-secreting tumors compared with control tumors. Together, these data suggest that IRDye800CW may be used to study the trafficking of T cells in a small animal model and may have potential as a shortterm reporter molecule for human immunotherapy studies.

AB - We describe a method to measure in vivo migration of human T cells by using the near-infrared (NIR) dye IRDye800CW, Labeling of Epstein-Barr virus-specific T cells with IRDye800CW did not affect viability, proliferation, or T cell function. Following tail vein injection into mice bearing subcutaneous tumors, the NIR signal could be measured in vivo at the tumor site. Analysis of tumors revealed T cell infiltration and an increased NIR signal, confirming T cell migration. To test specific migration with IRDye800CW, tumors were modified to express CCL5 to measure site-specific migration. The NIR signal was increased at CCL5-secreting tumors compared with control tumors. Together, these data suggest that IRDye800CW may be used to study the trafficking of T cells in a small animal model and may have potential as a shortterm reporter molecule for human immunotherapy studies.

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In vivo fluorescent optical imaging of cytotoxic T lymphocyte migration using IRDye800CW near-infrared dye

Abstract

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