In vivo fluorescent optical imaging of cytotoxic T lymphocyte migration using IRDye800CW near-infrared dye

Aaron E. Foster; Sunkuk Kwon; Shi Ke; An Lu; Karen Eldin; Eva Sevick-Muraca; Cliona M. Rooney

doi:10.1364/AO.47.005944

In vivo fluorescent optical imaging of cytotoxic T lymphocyte migration using IRDye800CW near-infrared dye

Aaron E. Foster, Sunkuk Kwon, Shi Ke, An Lu, Karen Eldin, Eva Sevick-Muraca, Cliona M. Rooney

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

We describe a method to measure in vivo migration of human T cells by using the near-infrared (NIR) dye IRDye800CW, Labeling of Epstein-Barr virus-specific T cells with IRDye800CW did not affect viability, proliferation, or T cell function. Following tail vein injection into mice bearing subcutaneous tumors, the NIR signal could be measured in vivo at the tumor site. Analysis of tumors revealed T cell infiltration and an increased NIR signal, confirming T cell migration. To test specific migration with IRDye800CW, tumors were modified to express CCL5 to measure site-specific migration. The NIR signal was increased at CCL5-secreting tumors compared with control tumors. Together, these data suggest that IRDye800CW may be used to study the trafficking of T cells in a small animal model and may have potential as a shortterm reporter molecule for human immunotherapy studies.

Original language	English (US)
Pages (from-to)	5944-5952
Number of pages	9
Journal	Applied Optics
Volume	47
Issue number	31
DOIs	https://doi.org/10.1364/AO.47.005944
State	Published - Nov 1 2008

ASJC Scopus subject areas

Atomic and Molecular Physics, and Optics
Engineering (miscellaneous)
Electrical and Electronic Engineering

Access to Document

10.1364/AO.47.005944

Cite this

@article{aa29cf90ee4847f1b58989990114beef,

title = "In vivo fluorescent optical imaging of cytotoxic T lymphocyte migration using IRDye800CW near-infrared dye",

abstract = "We describe a method to measure in vivo migration of human T cells by using the near-infrared (NIR) dye IRDye800CW, Labeling of Epstein-Barr virus-specific T cells with IRDye800CW did not affect viability, proliferation, or T cell function. Following tail vein injection into mice bearing subcutaneous tumors, the NIR signal could be measured in vivo at the tumor site. Analysis of tumors revealed T cell infiltration and an increased NIR signal, confirming T cell migration. To test specific migration with IRDye800CW, tumors were modified to express CCL5 to measure site-specific migration. The NIR signal was increased at CCL5-secreting tumors compared with control tumors. Together, these data suggest that IRDye800CW may be used to study the trafficking of T cells in a small animal model and may have potential as a shortterm reporter molecule for human immunotherapy studies.",

author = "Foster, {Aaron E.} and Sunkuk Kwon and Shi Ke and An Lu and Karen Eldin and Eva Sevick-Muraca and Rooney, {Cliona M.}",

year = "2008",

month = nov,

day = "1",

doi = "10.1364/AO.47.005944",

language = "English (US)",

volume = "47",

pages = "5944--5952",

journal = "Applied Optics",

issn = "1559-128X",

publisher = "The Optical Society",

number = "31",

}

TY - JOUR

T1 - In vivo fluorescent optical imaging of cytotoxic T lymphocyte migration using IRDye800CW near-infrared dye

AU - Foster, Aaron E.

AU - Kwon, Sunkuk

AU - Ke, Shi

AU - Lu, An

AU - Eldin, Karen

AU - Sevick-Muraca, Eva

AU - Rooney, Cliona M.

PY - 2008/11/1

Y1 - 2008/11/1

N2 - We describe a method to measure in vivo migration of human T cells by using the near-infrared (NIR) dye IRDye800CW, Labeling of Epstein-Barr virus-specific T cells with IRDye800CW did not affect viability, proliferation, or T cell function. Following tail vein injection into mice bearing subcutaneous tumors, the NIR signal could be measured in vivo at the tumor site. Analysis of tumors revealed T cell infiltration and an increased NIR signal, confirming T cell migration. To test specific migration with IRDye800CW, tumors were modified to express CCL5 to measure site-specific migration. The NIR signal was increased at CCL5-secreting tumors compared with control tumors. Together, these data suggest that IRDye800CW may be used to study the trafficking of T cells in a small animal model and may have potential as a shortterm reporter molecule for human immunotherapy studies.

AB - We describe a method to measure in vivo migration of human T cells by using the near-infrared (NIR) dye IRDye800CW, Labeling of Epstein-Barr virus-specific T cells with IRDye800CW did not affect viability, proliferation, or T cell function. Following tail vein injection into mice bearing subcutaneous tumors, the NIR signal could be measured in vivo at the tumor site. Analysis of tumors revealed T cell infiltration and an increased NIR signal, confirming T cell migration. To test specific migration with IRDye800CW, tumors were modified to express CCL5 to measure site-specific migration. The NIR signal was increased at CCL5-secreting tumors compared with control tumors. Together, these data suggest that IRDye800CW may be used to study the trafficking of T cells in a small animal model and may have potential as a shortterm reporter molecule for human immunotherapy studies.

UR - http://www.scopus.com/inward/record.url?scp=60649108303&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=60649108303&partnerID=8YFLogxK

U2 - 10.1364/AO.47.005944

DO - 10.1364/AO.47.005944

M3 - Article

C2 - 19122737

AN - SCOPUS:60649108303

VL - 47

SP - 5944

EP - 5952

JO - Applied Optics

JF - Applied Optics

SN - 1559-128X

IS - 31

ER -

In vivo fluorescent optical imaging of cytotoxic T lymphocyte migration using IRDye800CW near-infrared dye

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this