In vivo fluorescent optical imaging of cytotoxic T lymphocyte migration using IRDye800CW near-infrared dye

Aaron E. Foster, Sunkuk Kwon, Shi Ke, An Lu, Karen Eldin, Eva Sevick-Muraca, Cliona M. Rooney

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

We describe a method to measure in vivo migration of human T cells by using the near-infrared (NIR) dye IRDye800CW, Labeling of Epstein-Barr virus-specific T cells with IRDye800CW did not affect viability, proliferation, or T cell function. Following tail vein injection into mice bearing subcutaneous tumors, the NIR signal could be measured in vivo at the tumor site. Analysis of tumors revealed T cell infiltration and an increased NIR signal, confirming T cell migration. To test specific migration with IRDye800CW, tumors were modified to express CCL5 to measure site-specific migration. The NIR signal was increased at CCL5-secreting tumors compared with control tumors. Together, these data suggest that IRDye800CW may be used to study the trafficking of T cells in a small animal model and may have potential as a shortterm reporter molecule for human immunotherapy studies.

Original languageEnglish (US)
Pages (from-to)5944-5952
Number of pages9
JournalApplied Optics
Volume47
Issue number31
DOIs
StatePublished - Nov 1 2008

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Engineering (miscellaneous)
  • Electrical and Electronic Engineering

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