In vivo flow cytometer for real-time detection and quantification of circulating cells

J. Novak, I. Georgakoudi, X. Wei, A. Prossin, C. P. Lin

Research output: Contribution to journalArticle

167 Scopus citations

Abstract

An in vivo flow cytometer is developed that allows the real-time detection and quantification of circulating fluorescently labeled cells in live animals. A signal from a cell population of interest is recorded as the cells pass through a slit of light focused across a blood vessel. Confocal detection of the excited fluorescence allows continuous monitoring of labeled cells in the upper layers of scattering tissue, such as the skin. The device is used to characterize the in vivo kinetics of red and white blood cells circulating in the vasculatüre of the mouse ear. Potential applications in biology and medicine are discussed.

Original languageEnglish (US)
Pages (from-to)77-79
Number of pages3
JournalOptics Letters
Volume29
Issue number1
DOIs
StatePublished - Jan 1 2004

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

Fingerprint Dive into the research topics of 'In vivo flow cytometer for real-time detection and quantification of circulating cells'. Together they form a unique fingerprint.

Cite this