In vitro tubulogenesis of endothelial cells: Analysis of a bifurcation process controlled by a mechanical switch

Philippe Tracqui, Patrick Namy, Jacques Ohayon

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Abstract

The formation of new blood vessels in vivo is a multistep process in which sprouting endothelial cells (ECs) form tubes with lumina, these tubes being additionally organized as capillary networks. In vitro models of tubulogenesis have been developed to investigate this highly regulated multifactorial process, with special attention paid to the determinant role of mechanical interactions between ECs and the extracellular matrix (ECM). In agreement with experimental results obtained when culturing endothelial EAhy926 cells on fibrin gels, we defined theoretical thresholds between cellular traction and active cell migration along ECM strain fields above which tubulogenesis is induced.We additionally illustrated how mechanical factors may provide long-ranged positional information signals leading to localized network formation, thus providing an alternative view to the classical approach of morphogenesis based on gradients of diffusible morphogens.

Original languageEnglish (US)
Title of host publicationModeling and Simulation in Science, Engineering and Technology
PublisherSpringer Basel
Pages47-57
Number of pages11
DOIs
StatePublished - 2007

Publication series

NameModeling and Simulation in Science, Engineering and Technology
Volume38
ISSN (Print)2164-3679
ISSN (Electronic)2164-3725

Keywords

  • Extracellular matrix rheology
  • In vitro angiogenesis
  • Mechanical signalling
  • Network morphogenesis
  • Positional information

ASJC Scopus subject areas

  • Modeling and Simulation
  • Engineering(all)
  • Fluid Flow and Transfer Processes
  • Computational Mathematics

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