In vitro expression of natural mutants of human lecithin:cholesterol acyltransferase

S. J. Qu, H. Z. Fan, F. Blanco-Vaca, H. J. Pownall

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

Fish-eye disease (FED) and familial lecithin:cholesterol acyltransferase (LCAT) deficiency (FLD) are rare disorders of lipid metabolism linked to mutations in the LCAT gene. Eleven LCAT cDNA constructs associated with FED and FLD were prepared by site-directed mutagenesis and expressed in COS-6 cells. Analysis of total RNA from wild-type, FED, and FLD transfectants revealed that MI contained LCAT-specific mRNA. Western blot analysis demonstrated that all LCAT transfectants synthesized LCAT. Mean LCAT secretion by FED transfectants was slightly lower than secretion by wild- type transfectants, whereas secretion by FLD transfectants was much lower. The specific activities of FED and FLD LCAT against model high density lipoproteins were 6% and 11%, respectively, of wild-type activity. The ratios of the LCAT activities against low density lipoproteins to those against model high density lipoproteins decreased in the order FED mutants > FLD mutants - wild type. FED and FLD LCAT mutants are different: the former are more active against low density lipoproteins, and the latter are less secretion-competent. The greater reactivity of FED LCAT against low density lipoproteins may explain the relative mildness of the clinical manifestations of FED compared to those of FLD.

Original languageEnglish (US)
Pages (from-to)967-974
Number of pages8
JournalJournal of lipid research
Volume36
Issue number5
StatePublished - 1995

Keywords

  • cholesterol transport
  • high density lipoproteins
  • LCAT deficiency

ASJC Scopus subject areas

  • Endocrinology

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