In vitro evaluation of the role of humoral immunity against Bartonella henselae

M. C. Rodriguez-Barradas; J. C. Bandres; R. J. Hamill; J. Trial; J. E. Clarridge; R. E. Baughn; R. D. Rossen

doi:10.1128/iai.63.6.2367-2370.1995

In vitro evaluation of the role of humoral immunity against Bartonella henselae

M. C. Rodriguez-Barradas, J. C. Bandres, R. J. Hamill, J. Trial, J. E. Clarridge, R. E. Baughn, R. D. Rossen

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30 Scopus citations

Abstract

The contribution of humoral immunity against Bartonella henselae was evaluated by examining the in vitro bactericidal activity of sera and the ability of these microorganisms to activate complement and stimulate phagocytosis and an oxidative burst in polymorphonuclear leukocytes. The organism was killed by complement-mediated cytolysis. Complement activation preferentially proceeded by the alternative pathway. The presence of specific antibodies did not increase the serum bactericidal activity or complement activation. However, phagocytosis and the subsequent production of oxygen radicals, evaluated by flow cytometry, were significantly enhanced in the presence of bacteria previously opsonized with immune sera.

Original language	English (US)
Pages (from-to)	2367-2370
Number of pages	4
Journal	Infection and Immunity
Volume	63
Issue number	6
DOIs	https://doi.org/10.1128/iai.63.6.2367-2370.1995
State	Published - 1995

ASJC Scopus subject areas

Parasitology
Microbiology
Immunology
Infectious Diseases

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10.1128/iai.63.6.2367-2370.1995

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title = "In vitro evaluation of the role of humoral immunity against Bartonella henselae",

abstract = "The contribution of humoral immunity against Bartonella henselae was evaluated by examining the in vitro bactericidal activity of sera and the ability of these microorganisms to activate complement and stimulate phagocytosis and an oxidative burst in polymorphonuclear leukocytes. The organism was killed by complement-mediated cytolysis. Complement activation preferentially proceeded by the alternative pathway. The presence of specific antibodies did not increase the serum bactericidal activity or complement activation. However, phagocytosis and the subsequent production of oxygen radicals, evaluated by flow cytometry, were significantly enhanced in the presence of bacteria previously opsonized with immune sera.",

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AU - Rodriguez-Barradas, M. C.

AU - Bandres, J. C.

AU - Hamill, R. J.

AU - Trial, J.

AU - Clarridge, J. E.

AU - Baughn, R. E.

AU - Rossen, R. D.

PY - 1995

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AB - The contribution of humoral immunity against Bartonella henselae was evaluated by examining the in vitro bactericidal activity of sera and the ability of these microorganisms to activate complement and stimulate phagocytosis and an oxidative burst in polymorphonuclear leukocytes. The organism was killed by complement-mediated cytolysis. Complement activation preferentially proceeded by the alternative pathway. The presence of specific antibodies did not increase the serum bactericidal activity or complement activation. However, phagocytosis and the subsequent production of oxygen radicals, evaluated by flow cytometry, were significantly enhanced in the presence of bacteria previously opsonized with immune sera.

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In vitro evaluation of the role of humoral immunity against Bartonella henselae

Abstract

ASJC Scopus subject areas

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