TY - JOUR
T1 - In vitro evaluation of poly(caporlactone) grafted dextran (PGD) nanoparticles with cancer cell
AU - Prabu, P.
AU - Chaudhari, Atul A.
AU - Aryal, Santosh
AU - Dharmaraj, N.
AU - Park, S. Y.
AU - Kim, W. D.
AU - Kim, H. Y.
N1 - Funding Information:
Acknowledgement This work was supported by the Korean Research Foundation Grant founded by the Korean Government (MOEHRD) (The Center for Health Care Technology Development, Chonbuk National University, Jeonju 561-756 Republic of Korea) and Korean Government Project No: 10028211, through Ministry of Commerce, Industry and Energy Department.
Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2008/5
Y1 - 2008/5
N2 - This study dealt with the preparation and characterization of coumarin-6 loaded poly(caprolactone) grafted dextran (PGD) nanoparticles (NPs) and evaluation of cellular uptake by using human gastric cancer cell line (SNU-638), in vitro. The potential application of these PGD NPs for sustained drug delivery was evaluated by the quantification and localization of the cellular uptake of fluorescent PGD NPs. Coumarin-6 loaded PGD NPs were prepared by a modified oil/water emulsion technique and characterized by various physico-chemical methods such as, laser light scattering for particle size and size distribution, atomic force microscopy (AFM), zeta-potential and spectrofluorometry to identify the release of fluorescent molecules from the NPs. SNU-638 was used to measure the cellular uptake of fluorescent PGD NPs. Confocal laser scanning microscopic images clearly showed the internalization of NPs by the SNU-638 cells. Cell viability was assessed by treating the SNU-638 cells with PGD NPs for 48 h. The results reveal, that these biodegradable polymeric NPs holds promise in biomedical field as a carrier.
AB - This study dealt with the preparation and characterization of coumarin-6 loaded poly(caprolactone) grafted dextran (PGD) nanoparticles (NPs) and evaluation of cellular uptake by using human gastric cancer cell line (SNU-638), in vitro. The potential application of these PGD NPs for sustained drug delivery was evaluated by the quantification and localization of the cellular uptake of fluorescent PGD NPs. Coumarin-6 loaded PGD NPs were prepared by a modified oil/water emulsion technique and characterized by various physico-chemical methods such as, laser light scattering for particle size and size distribution, atomic force microscopy (AFM), zeta-potential and spectrofluorometry to identify the release of fluorescent molecules from the NPs. SNU-638 was used to measure the cellular uptake of fluorescent PGD NPs. Confocal laser scanning microscopic images clearly showed the internalization of NPs by the SNU-638 cells. Cell viability was assessed by treating the SNU-638 cells with PGD NPs for 48 h. The results reveal, that these biodegradable polymeric NPs holds promise in biomedical field as a carrier.
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U2 - 10.1007/s10856-007-3307-z
DO - 10.1007/s10856-007-3307-z
M3 - Article
C2 - 18040758
AN - SCOPUS:41149103366
SN - 0957-4530
VL - 19
SP - 2157
EP - 2163
JO - Journal of Materials Science: Materials in Medicine
JF - Journal of Materials Science: Materials in Medicine
IS - 5
ER -