In vitro evaluation of poly(caporlactone) grafted dextran (PGD) nanoparticles with cancer cell

P. Prabu, Atul A. Chaudhari, Santosh Aryal, N. Dharmaraj, S. Y. Park, W. D. Kim, H. Y. Kim

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

This study dealt with the preparation and characterization of coumarin-6 loaded poly(caprolactone) grafted dextran (PGD) nanoparticles (NPs) and evaluation of cellular uptake by using human gastric cancer cell line (SNU-638), in vitro. The potential application of these PGD NPs for sustained drug delivery was evaluated by the quantification and localization of the cellular uptake of fluorescent PGD NPs. Coumarin-6 loaded PGD NPs were prepared by a modified oil/water emulsion technique and characterized by various physico-chemical methods such as, laser light scattering for particle size and size distribution, atomic force microscopy (AFM), zeta-potential and spectrofluorometry to identify the release of fluorescent molecules from the NPs. SNU-638 was used to measure the cellular uptake of fluorescent PGD NPs. Confocal laser scanning microscopic images clearly showed the internalization of NPs by the SNU-638 cells. Cell viability was assessed by treating the SNU-638 cells with PGD NPs for 48 h. The results reveal, that these biodegradable polymeric NPs holds promise in biomedical field as a carrier.

Original languageEnglish (US)
Pages (from-to)2157-2163
Number of pages7
JournalJournal of Materials Science: Materials in Medicine
Volume19
Issue number5
DOIs
StatePublished - May 2008

ASJC Scopus subject areas

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Biomedical Engineering

Fingerprint Dive into the research topics of 'In vitro evaluation of poly(caporlactone) grafted dextran (PGD) nanoparticles with cancer cell'. Together they form a unique fingerprint.

Cite this