In vitro effectiveness of microspheres based on silk sericin and Chlorella vulgaris or Arthrospira platensis for wound healing applications

Elia Bari; Carla Renata Arciola; Barbara Vigani; Barbara Crivelli; Paola Moro; Giorgio Marrubini; Milena Sorrenti; Laura Catenacci; Giovanna Bruni; Theodora Chlapanidas; Enrico Lucarelli; Sara Perteghella; Maria Luisa Torre

doi:10.3390/ma10090983

In vitro effectiveness of microspheres based on silk sericin and Chlorella vulgaris or Arthrospira platensis for wound healing applications

Elia Bari, Carla Renata Arciola, Barbara Vigani, Barbara Crivelli, Paola Moro, Giorgio Marrubini, Milena Sorrenti, Laura Catenacci, Giovanna Bruni, Theodora Chlapanidas, Enrico Lucarelli, Sara Perteghella, Maria Luisa Torre

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33 Scopus citations

Abstract

Some natural compounds have recently been widely employed in wound healingapplications due to their biological properties. One such compound is sericin, which is producedby Bombix mori, while active polyphenols, polysaccharides and proteins are synthetized byChlorella vulgaris and Arthrospira platensis microalgae. Our hypothesis was that sericin, as an optimalbioactive polymeric carrier for microencapsulation process, could also improve the regenerative effectof the microalgae. A solvent-free extraction method and spray drying technique were combined toobtain five formulations, based on algal extracts (C. vulgaris and A. platensis, Chl and Art, respectively)or silk sericin (Ser) or their mixtures (Chl-Ser and Art-Ser). The spray drying was a suitable methodto produce microspheres with similar dimensions, characterized by collapsed morphology witha rough surface. Art and Art-Ser showed higher antioxidant properties than other formulations.All microspheres resulted in cytocompatibility on fibroblasts until 1.25 mg/mL and promoted cellmigration and the complete wound closure; this positive effect was further highlighted after treatmentwith Art and Art-Ser. To our surprize the combination of sericin to Art did not improve the microalgaeextract efficacy, at least in our experimental conditions.

Original language	English (US)
Article number	983
Journal	Materials
Volume	10
Issue number	9
DOIs	https://doi.org/10.3390/ma10090983
State	Published - Aug 23 2017

Keywords

Arthrospira platensis
Chlorella vulgaris
Microspheres
Silk sericin
Spray drying
Wound healing

ASJC Scopus subject areas

Materials Science(all)

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10.3390/ma10090983

Cite this

Bari, E., Arciola, C. R., Vigani, B., Crivelli, B., Moro, P., Marrubini, G., Sorrenti, M., Catenacci, L., Bruni, G., Chlapanidas, T., Lucarelli, E., Perteghella, S., & Torre, M. L. (2017). In vitro effectiveness of microspheres based on silk sericin and Chlorella vulgaris or Arthrospira platensis for wound healing applications. Materials, 10(9), [983]. https://doi.org/10.3390/ma10090983

Bari, E, Arciola, CR, Vigani, B, Crivelli, B, Moro, P, Marrubini, G, Sorrenti, M, Catenacci, L, Bruni, G, Chlapanidas, T, Lucarelli, E, Perteghella, S & Torre, ML 2017, 'In vitro effectiveness of microspheres based on silk sericin and Chlorella vulgaris or Arthrospira platensis for wound healing applications', Materials, vol. 10, no. 9, 983. https://doi.org/10.3390/ma10090983

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title = "In vitro effectiveness of microspheres based on silk sericin and Chlorella vulgaris or Arthrospira platensis for wound healing applications",

abstract = "Some natural compounds have recently been widely employed in wound healingapplications due to their biological properties. One such compound is sericin, which is producedby Bombix mori, while active polyphenols, polysaccharides and proteins are synthetized byChlorella vulgaris and Arthrospira platensis microalgae. Our hypothesis was that sericin, as an optimalbioactive polymeric carrier for microencapsulation process, could also improve the regenerative effectof the microalgae. A solvent-free extraction method and spray drying technique were combined toobtain five formulations, based on algal extracts (C. vulgaris and A. platensis, Chl and Art, respectively)or silk sericin (Ser) or their mixtures (Chl-Ser and Art-Ser). The spray drying was a suitable methodto produce microspheres with similar dimensions, characterized by collapsed morphology witha rough surface. Art and Art-Ser showed higher antioxidant properties than other formulations.All microspheres resulted in cytocompatibility on fibroblasts until 1.25 mg/mL and promoted cellmigration and the complete wound closure; this positive effect was further highlighted after treatmentwith Art and Art-Ser. To our surprize the combination of sericin to Art did not improve the microalgaeextract efficacy, at least in our experimental conditions.",

keywords = "Arthrospira platensis, Chlorella vulgaris, Microspheres, Silk sericin, Spray drying, Wound healing",

author = "Elia Bari and Arciola, {Carla Renata} and Barbara Vigani and Barbara Crivelli and Paola Moro and Giorgio Marrubini and Milena Sorrenti and Laura Catenacci and Giovanna Bruni and Theodora Chlapanidas and Enrico Lucarelli and Sara Perteghella and Torre, {Maria Luisa}",

note = "Funding Information: Acknowledgments: This work was supported by Fondazione Cariplo (MICROFLOWER, Project Id. 2014-0601). The authors thank Nembri Industrie Tessili S.r.l. (Capriolo (BS), Italy) for certified Bombyx mori cocoons, Sergio Schinelli and Dott. Mayra Paolillo (Drug Sciences Dept., Pavia University, Pavia, Italy) for willingness regarding the use of Synergy HT (BioTek, Swindon, UK). We are grateful to Ryan Rogers, University of Michigan, for editorial help. Publisher Copyright: {\textcopyright} 2017 by the authors.",

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AU - Bari, Elia

AU - Arciola, Carla Renata

AU - Vigani, Barbara

AU - Crivelli, Barbara

AU - Moro, Paola

AU - Marrubini, Giorgio

AU - Sorrenti, Milena

AU - Catenacci, Laura

AU - Bruni, Giovanna

AU - Chlapanidas, Theodora

AU - Lucarelli, Enrico

AU - Perteghella, Sara

AU - Torre, Maria Luisa

N1 - Funding Information: Acknowledgments: This work was supported by Fondazione Cariplo (MICROFLOWER, Project Id. 2014-0601). The authors thank Nembri Industrie Tessili S.r.l. (Capriolo (BS), Italy) for certified Bombyx mori cocoons, Sergio Schinelli and Dott. Mayra Paolillo (Drug Sciences Dept., Pavia University, Pavia, Italy) for willingness regarding the use of Synergy HT (BioTek, Swindon, UK). We are grateful to Ryan Rogers, University of Michigan, for editorial help. Publisher Copyright: © 2017 by the authors.

PY - 2017/8/23

Y1 - 2017/8/23

N2 - Some natural compounds have recently been widely employed in wound healingapplications due to their biological properties. One such compound is sericin, which is producedby Bombix mori, while active polyphenols, polysaccharides and proteins are synthetized byChlorella vulgaris and Arthrospira platensis microalgae. Our hypothesis was that sericin, as an optimalbioactive polymeric carrier for microencapsulation process, could also improve the regenerative effectof the microalgae. A solvent-free extraction method and spray drying technique were combined toobtain five formulations, based on algal extracts (C. vulgaris and A. platensis, Chl and Art, respectively)or silk sericin (Ser) or their mixtures (Chl-Ser and Art-Ser). The spray drying was a suitable methodto produce microspheres with similar dimensions, characterized by collapsed morphology witha rough surface. Art and Art-Ser showed higher antioxidant properties than other formulations.All microspheres resulted in cytocompatibility on fibroblasts until 1.25 mg/mL and promoted cellmigration and the complete wound closure; this positive effect was further highlighted after treatmentwith Art and Art-Ser. To our surprize the combination of sericin to Art did not improve the microalgaeextract efficacy, at least in our experimental conditions.

AB - Some natural compounds have recently been widely employed in wound healingapplications due to their biological properties. One such compound is sericin, which is producedby Bombix mori, while active polyphenols, polysaccharides and proteins are synthetized byChlorella vulgaris and Arthrospira platensis microalgae. Our hypothesis was that sericin, as an optimalbioactive polymeric carrier for microencapsulation process, could also improve the regenerative effectof the microalgae. A solvent-free extraction method and spray drying technique were combined toobtain five formulations, based on algal extracts (C. vulgaris and A. platensis, Chl and Art, respectively)or silk sericin (Ser) or their mixtures (Chl-Ser and Art-Ser). The spray drying was a suitable methodto produce microspheres with similar dimensions, characterized by collapsed morphology witha rough surface. Art and Art-Ser showed higher antioxidant properties than other formulations.All microspheres resulted in cytocompatibility on fibroblasts until 1.25 mg/mL and promoted cellmigration and the complete wound closure; this positive effect was further highlighted after treatmentwith Art and Art-Ser. To our surprize the combination of sericin to Art did not improve the microalgaeextract efficacy, at least in our experimental conditions.

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KW - Chlorella vulgaris

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KW - Silk sericin

KW - Spray drying

KW - Wound healing

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In vitro effectiveness of microspheres based on silk sericin and Chlorella vulgaris or Arthrospira platensis for wound healing applications

Abstract

Keywords

ASJC Scopus subject areas

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