TY - JOUR
T1 - In vitro effectiveness of microspheres based on silk sericin and Chlorella vulgaris or Arthrospira platensis for wound healing applications
AU - Bari, Elia
AU - Arciola, Carla Renata
AU - Vigani, Barbara
AU - Crivelli, Barbara
AU - Moro, Paola
AU - Marrubini, Giorgio
AU - Sorrenti, Milena
AU - Catenacci, Laura
AU - Bruni, Giovanna
AU - Chlapanidas, Theodora
AU - Lucarelli, Enrico
AU - Perteghella, Sara
AU - Torre, Maria Luisa
PY - 2017/8/23
Y1 - 2017/8/23
N2 - Some natural compounds have recently been widely employed in wound healingapplications due to their biological properties. One such compound is sericin, which is producedby Bombix mori, while active polyphenols, polysaccharides and proteins are synthetized byChlorella vulgaris and Arthrospira platensis microalgae. Our hypothesis was that sericin, as an optimalbioactive polymeric carrier for microencapsulation process, could also improve the regenerative effectof the microalgae. A solvent-free extraction method and spray drying technique were combined toobtain five formulations, based on algal extracts (C. vulgaris and A. platensis, Chl and Art, respectively)or silk sericin (Ser) or their mixtures (Chl-Ser and Art-Ser). The spray drying was a suitable methodto produce microspheres with similar dimensions, characterized by collapsed morphology witha rough surface. Art and Art-Ser showed higher antioxidant properties than other formulations.All microspheres resulted in cytocompatibility on fibroblasts until 1.25 mg/mL and promoted cellmigration and the complete wound closure; this positive effect was further highlighted after treatmentwith Art and Art-Ser. To our surprize the combination of sericin to Art did not improve the microalgaeextract efficacy, at least in our experimental conditions.
AB - Some natural compounds have recently been widely employed in wound healingapplications due to their biological properties. One such compound is sericin, which is producedby Bombix mori, while active polyphenols, polysaccharides and proteins are synthetized byChlorella vulgaris and Arthrospira platensis microalgae. Our hypothesis was that sericin, as an optimalbioactive polymeric carrier for microencapsulation process, could also improve the regenerative effectof the microalgae. A solvent-free extraction method and spray drying technique were combined toobtain five formulations, based on algal extracts (C. vulgaris and A. platensis, Chl and Art, respectively)or silk sericin (Ser) or their mixtures (Chl-Ser and Art-Ser). The spray drying was a suitable methodto produce microspheres with similar dimensions, characterized by collapsed morphology witha rough surface. Art and Art-Ser showed higher antioxidant properties than other formulations.All microspheres resulted in cytocompatibility on fibroblasts until 1.25 mg/mL and promoted cellmigration and the complete wound closure; this positive effect was further highlighted after treatmentwith Art and Art-Ser. To our surprize the combination of sericin to Art did not improve the microalgaeextract efficacy, at least in our experimental conditions.
KW - Arthrospira platensis
KW - Chlorella vulgaris
KW - Microspheres
KW - Silk sericin
KW - Spray drying
KW - Wound healing
UR - http://www.scopus.com/inward/record.url?scp=85028393941&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85028393941&partnerID=8YFLogxK
U2 - 10.3390/ma10090983
DO - 10.3390/ma10090983
M3 - Article
AN - SCOPUS:85028393941
VL - 10
JO - Materials
JF - Materials
SN - 1996-1944
IS - 9
M1 - 983
ER -