In vitro and in vivo two-photon luminescence imaging of single gold nanorods

Haifeng Wang, Terry B. Huff, Daniel A. Zweifel, Wei He, Philip S. Low, Alexander Wei, Ji Xin Cheng

Research output: Contribution to journalArticlepeer-review

942 Scopus citations


Gold nanorods excited at 830 nm on a far-field laser-scanning microscope produced strong two-photon luminescence (TPL) intensities, with a cos 4 dependence on the incident polarization. The TPL excitation spectrum can be superimposed onto the longitudinal plasmon band, indicating a plasmon-enhanced two-photon absorption cross section. The TPL signal from a single nanorod is 58 times that of the two-photon fluorescence signal from a single rhodamine molecule. The application of gold nanorods as TPL imaging agents is demonstrated by in vivo imaging of single nanorods flowing in mouse ear blood vessels.

Original languageEnglish (US)
Pages (from-to)15752-15756
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number44
StatePublished - Nov 1 2005


  • In vivo imaging
  • Multiphoton
  • Nonlinear optics
  • Plasmon resonance

ASJC Scopus subject areas

  • Genetics
  • General


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