In vitro and in vivo two-photon luminescence imaging of single gold nanorods

Haifeng Wang; Terry B. Huff; Daniel A. Zweifel; Wei He; Philip S. Low; Alexander Wei; Ji Xin Cheng

doi:10.1073/pnas.0504892102

In vitro and in vivo two-photon luminescence imaging of single gold nanorods

Haifeng Wang, Terry B. Huff, Daniel A. Zweifel, Wei He, Philip S. Low, Alexander Wei, Ji Xin Cheng

Research output: Contribution to journal › Article › peer-review

874 Scopus citations

Abstract

Gold nanorods excited at 830 nm on a far-field laser-scanning microscope produced strong two-photon luminescence (TPL) intensities, with a cos ⁴ dependence on the incident polarization. The TPL excitation spectrum can be superimposed onto the longitudinal plasmon band, indicating a plasmon-enhanced two-photon absorption cross section. The TPL signal from a single nanorod is 58 times that of the two-photon fluorescence signal from a single rhodamine molecule. The application of gold nanorods as TPL imaging agents is demonstrated by in vivo imaging of single nanorods flowing in mouse ear blood vessels.

Original language	English (US)
Pages (from-to)	15752-15756
Number of pages	5
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	102
Issue number	44
DOIs	https://doi.org/10.1073/pnas.0504892102
State	Published - Nov 1 2005

Keywords

In vivo imaging
Multiphoton
Nonlinear optics
Plasmon resonance

ASJC Scopus subject areas

Genetics
General

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10.1073/pnas.0504892102

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In vitro and in vivo two-photon luminescence imaging of single gold nanorods. / Wang, Haifeng; Huff, Terry B.; Zweifel, Daniel A.; He, Wei; Low, Philip S.; Wei, Alexander; Cheng, Ji Xin.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, No. 44, 01.11.2005, p. 15752-15756.

Research output: Contribution to journal › Article › peer-review

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title = "In vitro and in vivo two-photon luminescence imaging of single gold nanorods",

abstract = "Gold nanorods excited at 830 nm on a far-field laser-scanning microscope produced strong two-photon luminescence (TPL) intensities, with a cos 4 dependence on the incident polarization. The TPL excitation spectrum can be superimposed onto the longitudinal plasmon band, indicating a plasmon-enhanced two-photon absorption cross section. The TPL signal from a single nanorod is 58 times that of the two-photon fluorescence signal from a single rhodamine molecule. The application of gold nanorods as TPL imaging agents is demonstrated by in vivo imaging of single nanorods flowing in mouse ear blood vessels.",

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AU - Huff, Terry B.

AU - Zweifel, Daniel A.

AU - He, Wei

AU - Low, Philip S.

AU - Wei, Alexander

AU - Cheng, Ji Xin

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KW - In vivo imaging

KW - Multiphoton

KW - Nonlinear optics

KW - Plasmon resonance

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In vitro and in vivo two-photon luminescence imaging of single gold nanorods

Abstract

Keywords

ASJC Scopus subject areas

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