In vitro- and in vivo-induced transgene expression in human embryonic stem cells and derivatives

Xiaofeng Xia, Melvin Ayala, Benjamin R. Thiede, Su Chun Zhang

Research output: Contribution to journalArticle

24 Scopus citations

Abstract

The use of human embryonic stem cells (hESCs) as a research and therapeutic tool will be facilitated by conditional gene expression. Here, we report drug-induced transgene expression, both in vitro and in vivo, from a tet-on hESC line with >95% purity. Using green fluorescent protein as an indicator, we demonstrated that the tet-on system allowed a tight control of the gene expression in both undifferentiated hESCs and differentiated cells of the three germ layers. More importantly, after the cells were transplanted into animals, the gene expression remained to be regulated by an orally administered drug. These results provide a technical basis for regulation of gene expression in hESCs and derivatives in vitro and in vivo.

Original languageEnglish (US)
Pages (from-to)525-533
Number of pages9
JournalSTEM CELLS
Volume26
Issue number2
DOIs
StatePublished - Feb 2008

Keywords

  • Gene delivery systems in vivo or in vitro
  • Gene modification
  • Human embryonic stem cell
  • Inducible gene expression

ASJC Scopus subject areas

  • Cell Biology

Fingerprint Dive into the research topics of 'In vitro- and in vivo-induced transgene expression in human embryonic stem cells and derivatives'. Together they form a unique fingerprint.

Cite this