TY - JOUR
T1 - In vitro- and in vivo-induced transgene expression in human embryonic stem cells and derivatives
AU - Xia, Xiaofeng
AU - Ayala, Melvin
AU - Thiede, Benjamin R.
AU - Zhang, Su Chun
N1 - Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2008/2
Y1 - 2008/2
N2 - The use of human embryonic stem cells (hESCs) as a research and therapeutic tool will be facilitated by conditional gene expression. Here, we report drug-induced transgene expression, both in vitro and in vivo, from a tet-on hESC line with >95% purity. Using green fluorescent protein as an indicator, we demonstrated that the tet-on system allowed a tight control of the gene expression in both undifferentiated hESCs and differentiated cells of the three germ layers. More importantly, after the cells were transplanted into animals, the gene expression remained to be regulated by an orally administered drug. These results provide a technical basis for regulation of gene expression in hESCs and derivatives in vitro and in vivo.
AB - The use of human embryonic stem cells (hESCs) as a research and therapeutic tool will be facilitated by conditional gene expression. Here, we report drug-induced transgene expression, both in vitro and in vivo, from a tet-on hESC line with >95% purity. Using green fluorescent protein as an indicator, we demonstrated that the tet-on system allowed a tight control of the gene expression in both undifferentiated hESCs and differentiated cells of the three germ layers. More importantly, after the cells were transplanted into animals, the gene expression remained to be regulated by an orally administered drug. These results provide a technical basis for regulation of gene expression in hESCs and derivatives in vitro and in vivo.
KW - Gene delivery systems in vivo or in vitro
KW - Gene modification
KW - Human embryonic stem cell
KW - Inducible gene expression
UR - http://www.scopus.com/inward/record.url?scp=40949165288&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=40949165288&partnerID=8YFLogxK
U2 - 10.1634/stemcells.2007-0710
DO - 10.1634/stemcells.2007-0710
M3 - Article
C2 - 18032700
AN - SCOPUS:40949165288
VL - 26
SP - 525
EP - 533
JO - STEM CELLS
JF - STEM CELLS
SN - 1066-5099
IS - 2
ER -