Abstract
The use of human embryonic stem cells (hESCs) as a research and therapeutic tool will be facilitated by conditional gene expression. Here, we report drug-induced transgene expression, both in vitro and in vivo, from a tet-on hESC line with >95% purity. Using green fluorescent protein as an indicator, we demonstrated that the tet-on system allowed a tight control of the gene expression in both undifferentiated hESCs and differentiated cells of the three germ layers. More importantly, after the cells were transplanted into animals, the gene expression remained to be regulated by an orally administered drug. These results provide a technical basis for regulation of gene expression in hESCs and derivatives in vitro and in vivo.
Original language | English (US) |
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Pages (from-to) | 525-533 |
Number of pages | 9 |
Journal | STEM CELLS |
Volume | 26 |
Issue number | 2 |
DOIs | |
State | Published - Feb 2008 |
Keywords
- Gene delivery systems in vivo or in vitro
- Gene modification
- Human embryonic stem cell
- Inducible gene expression
ASJC Scopus subject areas
- Cell Biology