In the Murine and Bovine Maternal Mammary Gland Signal Transducer and Activator of Transcription 3 is Activated in Clusters of Epithelial Cells around the Day of Birth

Laura J.A. Hardwick; Benjamin P. Davies; Sara Pensa; Maedee Burge-Rogers; Claire Davies; André Figueiredo Baptista; Robert Knott; Ian S. McCrone; Eleonora Po; Benjamin W. Strugnell; Katie Waine; Paul Wood; Walid T. Khaled; Huw D. Summers; Paul Rees; John W. Wills; Katherine Hughes

doi:10.1007/s10911-024-09561-5

In the Murine and Bovine Maternal Mammary Gland Signal Transducer and Activator of Transcription 3 is Activated in Clusters of Epithelial Cells around the Day of Birth

Laura J.A. Hardwick, Benjamin P. Davies, Sara Pensa, Maedee Burge-Rogers, Claire Davies, André Figueiredo Baptista, Robert Knott, Ian S. McCrone, Eleonora Po, Benjamin W. Strugnell, Katie Waine, Paul Wood, Walid T. Khaled, Huw D. Summers, Paul Rees, John W. Wills, Katherine Hughes

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Signal transducers and activators of transcription (STAT) proteins regulate mammary development. Here we investigate the expression of phosphorylated STAT3 (pSTAT3) in the mouse and cow around the day of birth. We present localised colocation analysis, applicable to other mammary studies requiring identification of spatially congregated events. We demonstrate that pSTAT3-positive events are multifocally clustered in a non-random and statistically significant fashion. Arginase-1 expressing cells, consistent with macrophages, exhibit distinct clustering within the periparturient mammary gland. These findings represent a new facet of mammary STAT3 biology, and point to the presence of mammary sub-microenvironments.

Original language	English (US)
Article number	10
Pages (from-to)	10
Journal	Journal of Mammary Gland Biology and Neoplasia
Volume	29
Issue number	1
DOIs	https://doi.org/10.1007/s10911-024-09561-5
State	Published - May 9 2024

Keywords

Macrophage
Mammary
Parturition
Spatial statistics
STAT3
Udder
Epithelial Cells/metabolism
Phosphorylation
Signal Transduction
Pregnancy
STAT3 Transcription Factor/metabolism
Animals
Mammary Glands, Animal/metabolism
Cattle
Female
Mice
Parturition/physiology

ASJC Scopus subject areas

Oncology
Cancer Research

Access to Document

10.1007/s10911-024-09561-5

Cite this

Hardwick, L. J. A., Davies, B. P., Pensa, S., Burge-Rogers, M., Davies, C., Baptista, A. F., Knott, R., S. McCrone, I., Po, E., Strugnell, B. W., Waine, K., Wood, P., Khaled, W. T., Summers, H. D., Rees, P., Wills, J. W., & Hughes, K. (2024). In the Murine and Bovine Maternal Mammary Gland Signal Transducer and Activator of Transcription 3 is Activated in Clusters of Epithelial Cells around the Day of Birth. Journal of Mammary Gland Biology and Neoplasia, 29(1), 10. Article 10. https://doi.org/10.1007/s10911-024-09561-5

In the Murine and Bovine Maternal Mammary Gland Signal Transducer and Activator of Transcription 3 is Activated in Clusters of Epithelial Cells around the Day of Birth. / Hardwick, Laura J.A.; Davies, Benjamin P.; Pensa, Sara et al.
In: Journal of Mammary Gland Biology and Neoplasia, Vol. 29, No. 1, 10, 09.05.2024, p. 10.

Research output: Contribution to journal › Article › peer-review

Hardwick, LJA, Davies, BP, Pensa, S, Burge-Rogers, M, Davies, C, Baptista, AF, Knott, R, S. McCrone, I, Po, E, Strugnell, BW, Waine, K, Wood, P, Khaled, WT, Summers, HD, Rees, P, Wills, JW & Hughes, K 2024, 'In the Murine and Bovine Maternal Mammary Gland Signal Transducer and Activator of Transcription 3 is Activated in Clusters of Epithelial Cells around the Day of Birth', Journal of Mammary Gland Biology and Neoplasia, vol. 29, no. 1, 10, pp. 10. https://doi.org/10.1007/s10911-024-09561-5

@article{b31ea033b2ce49848bacce66689a3284,

title = "In the Murine and Bovine Maternal Mammary Gland Signal Transducer and Activator of Transcription 3 is Activated in Clusters of Epithelial Cells around the Day of Birth",

abstract = "Signal transducers and activators of transcription (STAT) proteins regulate mammary development. Here we investigate the expression of phosphorylated STAT3 (pSTAT3) in the mouse and cow around the day of birth. We present localised colocation analysis, applicable to other mammary studies requiring identification of spatially congregated events. We demonstrate that pSTAT3-positive events are multifocally clustered in a non-random and statistically significant fashion. Arginase-1 expressing cells, consistent with macrophages, exhibit distinct clustering within the periparturient mammary gland. These findings represent a new facet of mammary STAT3 biology, and point to the presence of mammary sub-microenvironments.",

keywords = "Macrophage, Mammary, Parturition, Spatial statistics, STAT3, Udder, Epithelial Cells/metabolism, Phosphorylation, Signal Transduction, Pregnancy, STAT3 Transcription Factor/metabolism, Animals, Mammary Glands, Animal/metabolism, Cattle, Female, Mice, Parturition/physiology",

author = "Hardwick, {Laura J.A.} and Davies, {Benjamin P.} and Sara Pensa and Maedee Burge-Rogers and Claire Davies and Baptista, {Andr{\'e} Figueiredo} and Robert Knott and {S. McCrone}, Ian and Eleonora Po and Strugnell, {Benjamin W.} and Katie Waine and Paul Wood and Khaled, {Walid T.} and Summers, {Huw D.} and Paul Rees and Wills, {John W.} and Katherine Hughes",

note = "Publisher Copyright: {\textcopyright} The Author(s) 2024.",

year = "2024",

month = may,

day = "9",

doi = "10.1007/s10911-024-09561-5",

language = "English (US)",

volume = "29",

pages = "10",

journal = "Journal of Mammary Gland Biology and Neoplasia",

issn = "1083-3021",

publisher = "Springer",

number = "1",

}

TY - JOUR

T1 - In the Murine and Bovine Maternal Mammary Gland Signal Transducer and Activator of Transcription 3 is Activated in Clusters of Epithelial Cells around the Day of Birth

AU - Hardwick, Laura J.A.

AU - Davies, Benjamin P.

AU - Pensa, Sara

AU - Burge-Rogers, Maedee

AU - Davies, Claire

AU - Baptista, André Figueiredo

AU - Knott, Robert

AU - S. McCrone, Ian

AU - Po, Eleonora

AU - Strugnell, Benjamin W.

AU - Waine, Katie

AU - Wood, Paul

AU - Khaled, Walid T.

AU - Summers, Huw D.

AU - Rees, Paul

AU - Wills, John W.

AU - Hughes, Katherine

N1 - Publisher Copyright: © The Author(s) 2024.

PY - 2024/5/9

Y1 - 2024/5/9

N2 - Signal transducers and activators of transcription (STAT) proteins regulate mammary development. Here we investigate the expression of phosphorylated STAT3 (pSTAT3) in the mouse and cow around the day of birth. We present localised colocation analysis, applicable to other mammary studies requiring identification of spatially congregated events. We demonstrate that pSTAT3-positive events are multifocally clustered in a non-random and statistically significant fashion. Arginase-1 expressing cells, consistent with macrophages, exhibit distinct clustering within the periparturient mammary gland. These findings represent a new facet of mammary STAT3 biology, and point to the presence of mammary sub-microenvironments.

AB - Signal transducers and activators of transcription (STAT) proteins regulate mammary development. Here we investigate the expression of phosphorylated STAT3 (pSTAT3) in the mouse and cow around the day of birth. We present localised colocation analysis, applicable to other mammary studies requiring identification of spatially congregated events. We demonstrate that pSTAT3-positive events are multifocally clustered in a non-random and statistically significant fashion. Arginase-1 expressing cells, consistent with macrophages, exhibit distinct clustering within the periparturient mammary gland. These findings represent a new facet of mammary STAT3 biology, and point to the presence of mammary sub-microenvironments.

KW - Macrophage

KW - Mammary

KW - Parturition

KW - Spatial statistics

KW - STAT3

KW - Udder

KW - Epithelial Cells/metabolism

KW - Phosphorylation

KW - Signal Transduction

KW - Pregnancy

KW - STAT3 Transcription Factor/metabolism

KW - Animals

KW - Mammary Glands, Animal/metabolism

KW - Cattle

KW - Female

KW - Mice

KW - Parturition/physiology

UR - http://www.scopus.com/inward/record.url?scp=85192560093&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85192560093&partnerID=8YFLogxK

U2 - 10.1007/s10911-024-09561-5

DO - 10.1007/s10911-024-09561-5

M3 - Article

C2 - 38722417

AN - SCOPUS:85192560093

SN - 1083-3021

VL - 29

SP - 10

JO - Journal of Mammary Gland Biology and Neoplasia

JF - Journal of Mammary Gland Biology and Neoplasia

IS - 1

M1 - 10

ER -

In the Murine and Bovine Maternal Mammary Gland Signal Transducer and Activator of Transcription 3 is Activated in Clusters of Epithelial Cells around the Day of Birth

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this