In Situ Transforming RNA Nanovaccines from Polyethylenimine Functionalized Graphene Oxide Hydrogel for Durable Cancer Immunotherapy

Yue Yin, Xiaoyang Li, Haixia Ma, Jie Zhang, Di Yu, Ruifang Zhao, Shengji Yu, Guangjun Nie, Hai Wang

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

Messenger RNA (mRNA) vaccine is a promising candidate in cancer immunotherapy as it can encode tumor-associated antigens with an excellent safety profile. Unfortunately, the inherent instability of RNA and translational efficiency are major limitations of RNA vaccine. Here, we report an injectable hydrogel formed with graphene oxide (GO) and polyethylenimine (PEI), which can generate mRNA (ovalbumin, a model antigen) and adjuvants (R848)-laden nanovaccines for at least 30 days after subcutaneous injection. The released nanovaccines can protect the mRNA from degradation and confer targeted delivering capacity to lymph nodes. The data show that this transformable hydrogel can significantly increase the number of antigen-specific CD8+ T cells and subsequently inhibit the tumor growth with only one treatment. Meanwhile, this hydrogel can generate an antigen specific antibody in the serum which in turn prevents the occurrence of metastasis. Collectively, these results demonstrate the potential of the PEI-functionalized GO transformable hydrogel for effective cancer immunotherapy.

Original languageEnglish (US)
Pages (from-to)2224-2231
Number of pages8
JournalNano Letters
Volume21
Issue number5
DOIs
StatePublished - Mar 10 2021

Keywords

  • RNA vaccine
  • graphene oxide
  • immunotherapy
  • long-term release
  • polyethylenimine

ASJC Scopus subject areas

  • Bioengineering
  • Chemistry(all)
  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanical Engineering

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