In situ replication of immediate dendritic cell (DC) precursors contributes to conventional DC homeostasis in lymphoid tissue

Jim Diao, Erin Winter, Claude Cantin, Wenhao Chen, Luoling Xu, David Kelvin, James Phillips, Mark S. Cattral

Research output: Contribution to journalArticle

72 Scopus citations

Abstract

The developmental biology of dendritic cells (DC) under physiological conditions remains unclear. In this study, we show that mouse CD11c+ MHC class II-lineage- cells are immediate precursors of conventional DC and are widely distributed in both bone marrow and lymphoid tissues. These precursors have a high clonal efficiency, and when cocultured on a supportive stromal monolayer or adoptively transferred in vivo, generate a population CD11c+MHC class II+ DC that retain limited proliferation capacity. During steady state conditions, a small proportion of immediate DC precursors (DCp) and DCs are dividing actively in bone marrow and spleen. Cytokines that initiate and support proliferation of immediate DCp were defined. Collectively, our findings provide evidence of a distinct development pathway for conventional DC in both bone marrow and lymphoid tissues and highlight the importance of in situ replication of immediate DCp and DC in maintaining conventional DC populations.

Original languageEnglish (US)
Pages (from-to)7196-7206
Number of pages11
JournalJournal of Immunology
Volume176
Issue number12
DOIs
StatePublished - Jun 15 2006

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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