TY - JOUR
T1 - In Situ Reductive Synthesis of Structural Supported Gold Nanorods in Porous Silicon Particles for Multifunctional Nanovectors
AU - Zhu, Guixian
AU - Liu, Jen Tsai
AU - Wang, Yuzhen
AU - Zhang, Dechen
AU - Guo, Yi
AU - Tasciotti, Ennio
AU - Hu, Zhongbo
AU - Liu, Xuewu
N1 - Funding Information:
The authors acknowledge financial support from the following sources: Grants NIH U54CA143837 (CTO, PS-OC), NIH U54CA151668-01 (TCCN, CCNE), DOD W81XWH-12-1-0414, and DOD W81XWH-10-2-0125, National Natural Science Foundation of China Grant 81571791, and Houston Methodist Research Institute.
Publisher Copyright:
© 2016 American Chemical Society.
PY - 2016/5/11
Y1 - 2016/5/11
N2 - Porous silicon nanodisks (PSD) were fabricated by the combination of photolithography and electrochemical etching of silicon. By using PSD as a reducing agent, gold nanorods (AuNR) were in situ synthesized in the nanopores of PSD, forming PSD-supported-AuNR (PSD/AuNR) hybrid particles. The formation mechanism of AuNR in porous silicon (pSi) was revealed by exploring the role of pSi reducibility and each chemical in the reaction. With the PSD support, AuNR exhibited a stable morphology without toxic surface ligands (CTAB). The PSD/AuNR hybrid particles showed enhanced plasmonic property compared to free AuNR. Because high-density "hot spots" can be generated by controlling the distribution of AuNR supported in PSD, surface-enhanced raman scattering (SERS) using PSD/AuNR as particle substrates was demonstrated. A multifunctional vector, PSD/AuNR/DOX, composed of doxorubicin (DOX)-loaded PSD/AuNR capped with agarose (agar), was developed for highly efficient, combinatorial cancer treatment. Their therapeutic efficacy was examined using two pancreatic cancer cell lines, PANC-1 and MIA PaCa-2. PSD/AuNR/DOX (20 μg Au and 1.25 μg DOX/mL) effectively destroyed these cells under near-IR laser irradiation (810 nm, 15 J·cm-2 power, 90 s). Overall, we envision that PSD/AuNR may be a promising injectable, multifunctional nanovector for biomedical application.
AB - Porous silicon nanodisks (PSD) were fabricated by the combination of photolithography and electrochemical etching of silicon. By using PSD as a reducing agent, gold nanorods (AuNR) were in situ synthesized in the nanopores of PSD, forming PSD-supported-AuNR (PSD/AuNR) hybrid particles. The formation mechanism of AuNR in porous silicon (pSi) was revealed by exploring the role of pSi reducibility and each chemical in the reaction. With the PSD support, AuNR exhibited a stable morphology without toxic surface ligands (CTAB). The PSD/AuNR hybrid particles showed enhanced plasmonic property compared to free AuNR. Because high-density "hot spots" can be generated by controlling the distribution of AuNR supported in PSD, surface-enhanced raman scattering (SERS) using PSD/AuNR as particle substrates was demonstrated. A multifunctional vector, PSD/AuNR/DOX, composed of doxorubicin (DOX)-loaded PSD/AuNR capped with agarose (agar), was developed for highly efficient, combinatorial cancer treatment. Their therapeutic efficacy was examined using two pancreatic cancer cell lines, PANC-1 and MIA PaCa-2. PSD/AuNR/DOX (20 μg Au and 1.25 μg DOX/mL) effectively destroyed these cells under near-IR laser irradiation (810 nm, 15 J·cm-2 power, 90 s). Overall, we envision that PSD/AuNR may be a promising injectable, multifunctional nanovector for biomedical application.
KW - SERS
KW - on-site synthesis
KW - photothermal therapy
KW - plasmonic gold nanorod
KW - porous silicon particles
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U2 - 10.1021/acsami.6b03008
DO - 10.1021/acsami.6b03008
M3 - Article
C2 - 27123698
AN - SCOPUS:84974809140
SN - 1944-8244
VL - 8
SP - 11881
EP - 11891
JO - ACS Applied Materials and Interfaces
JF - ACS Applied Materials and Interfaces
IS - 18
ER -