TY - JOUR
T1 - In situ observation of C60(C(COOH)2)2 interacting with living cells using fluorescence microscopy
AU - Ye, Chang
AU - Chen, Chunying
AU - Chen, Zhen
AU - Meng, Huan
AU - Xing, Li
AU - Jiang, Yaxin
AU - Yuan, Hui
AU - Xing, Gengmei
AU - Zhao, Feng
AU - Zhao, Yuliang
AU - Chai, Zhifang
AU - Fang, Xiaohong
AU - Han, Dong
AU - Chen, Long
AU - Wang, Chen
AU - Wei, Taotao
PY - 2006/5
Y1 - 2006/5
N2 - The interactions between nanoparticles and living cells were investigated by an imaging technique of fluorescence microscopy. For this purpose, the C 60 derivative C60(C(COOH)2)2, a therapeutic agent for degeneration diseases of central nervous system, was synthesized, purified and characterized. Its interaction with the living cell and penetration of the cellular membrane were in situ studied using the real time imaging technique, and its potential cytotoxicity was also examined by flow cytometry. The results indicate that C60(C(COOH)2) 2 can easily enter cells, and is mainly located in cytoplasm by fluorescein labeling. Furthermore, C60(C(COOH)2) 2 can carry the molecule that cannot cross cellular membranes into cells, because fluorescein compound itself cannot enter the cell or adhere to membrane. At concentrations ranging from 1 × 10-2 to 1 × 102 mg/L, C60(C(COOH)2)2 does not show any detectable cytotoxicity.
AB - The interactions between nanoparticles and living cells were investigated by an imaging technique of fluorescence microscopy. For this purpose, the C 60 derivative C60(C(COOH)2)2, a therapeutic agent for degeneration diseases of central nervous system, was synthesized, purified and characterized. Its interaction with the living cell and penetration of the cellular membrane were in situ studied using the real time imaging technique, and its potential cytotoxicity was also examined by flow cytometry. The results indicate that C60(C(COOH)2) 2 can easily enter cells, and is mainly located in cytoplasm by fluorescein labeling. Furthermore, C60(C(COOH)2) 2 can carry the molecule that cannot cross cellular membranes into cells, because fluorescein compound itself cannot enter the cell or adhere to membrane. At concentrations ranging from 1 × 10-2 to 1 × 102 mg/L, C60(C(COOH)2)2 does not show any detectable cytotoxicity.
KW - Cross cellular membrane
KW - Fluorescence labeling
KW - Fluorescence microscopy
KW - Fullerene derivative C (C(COOH))
KW - Real time imaging
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U2 - 10.1007/s11434-006-1060-1
DO - 10.1007/s11434-006-1060-1
M3 - Article
AN - SCOPUS:33748919846
VL - 51
SP - 1060
EP - 1064
JO - Chinese Science Bulletin
JF - Chinese Science Bulletin
SN - 1001-6538
IS - 9
ER -