In situ observation of C60(C(COOH)2)2 interacting with living cells using fluorescence microscopy

Chang Ye; Chunying Chen; Zhen Chen; Huan Meng; Li Xing; Yaxin Jiang; Hui Yuan; Gengmei Xing; Feng Zhao; Yuliang Zhao; Zhifang Chai; Xiaohong Fang; Dong Han; Long Chen; Chen Wang; Taotao Wei

doi:10.1007/s11434-006-1060-1

In situ observation of C₆₀(C(COOH)₂)₂ interacting with living cells using fluorescence microscopy

Chang Ye, Chunying Chen, Zhen Chen, Huan Meng, Li Xing, Yaxin Jiang, Hui Yuan, Gengmei Xing, Feng Zhao, Yuliang Zhao, Zhifang Chai, Xiaohong Fang, Dong Han, Long Chen, Chen Wang, Taotao Wei

Research output: Contribution to journal › Article

23 Scopus citations

Abstract

The interactions between nanoparticles and living cells were investigated by an imaging technique of fluorescence microscopy. For this purpose, the C ₆₀ derivative C₆₀(C(COOH)₂)₂, a therapeutic agent for degeneration diseases of central nervous system, was synthesized, purified and characterized. Its interaction with the living cell and penetration of the cellular membrane were in situ studied using the real time imaging technique, and its potential cytotoxicity was also examined by flow cytometry. The results indicate that C₆₀(C(COOH)₂) ₂ can easily enter cells, and is mainly located in cytoplasm by fluorescein labeling. Furthermore, C₆₀(C(COOH)₂) ₂ can carry the molecule that cannot cross cellular membranes into cells, because fluorescein compound itself cannot enter the cell or adhere to membrane. At concentrations ranging from 1 × 10^-2 to 1 × 10² mg/L, C₆₀(C(COOH)₂)₂ does not show any detectable cytotoxicity.

Original language	English (US)
Pages (from-to)	1060-1064
Number of pages	5
Journal	Chinese Science Bulletin
Volume	51
Issue number	9
DOIs	https://doi.org/10.1007/s11434-006-1060-1
State	Published - May 2006

Keywords

Cross cellular membrane
Fluorescence labeling
Fluorescence microscopy
Fullerene derivative C (C(COOH))
Real time imaging

ASJC Scopus subject areas

General

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In situ observation of C₆₀(C(COOH)₂)₂ interacting with living cells using fluorescence microscopy. / Ye, Chang; Chen, Chunying; Chen, Zhen; Meng, Huan; Xing, Li; Jiang, Yaxin; Yuan, Hui; Xing, Gengmei; Zhao, Feng; Zhao, Yuliang; Chai, Zhifang; Fang, Xiaohong; Han, Dong; Chen, Long; Wang, Chen; Wei, Taotao.

In: Chinese Science Bulletin, Vol. 51, No. 9, 05.2006, p. 1060-1064.

Research output: Contribution to journal › Article

Ye, Chang ; Chen, Chunying ; Chen, Zhen ; Meng, Huan ; Xing, Li ; Jiang, Yaxin ; Yuan, Hui ; Xing, Gengmei ; Zhao, Feng ; Zhao, Yuliang ; Chai, Zhifang ; Fang, Xiaohong ; Han, Dong ; Chen, Long ; Wang, Chen ; Wei, Taotao. / In situ observation of C₆₀(C(COOH)₂)₂ interacting with living cells using fluorescence microscopy. In: Chinese Science Bulletin. 2006 ; Vol. 51, No. 9. pp. 1060-1064.

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title = "In situ observation of C60(C(COOH)2)2 interacting with living cells using fluorescence microscopy",

abstract = "The interactions between nanoparticles and living cells were investigated by an imaging technique of fluorescence microscopy. For this purpose, the C 60 derivative C60(C(COOH)2)2, a therapeutic agent for degeneration diseases of central nervous system, was synthesized, purified and characterized. Its interaction with the living cell and penetration of the cellular membrane were in situ studied using the real time imaging technique, and its potential cytotoxicity was also examined by flow cytometry. The results indicate that C60(C(COOH)2) 2 can easily enter cells, and is mainly located in cytoplasm by fluorescein labeling. Furthermore, C60(C(COOH)2) 2 can carry the molecule that cannot cross cellular membranes into cells, because fluorescein compound itself cannot enter the cell or adhere to membrane. At concentrations ranging from 1 × 10-2 to 1 × 102 mg/L, C60(C(COOH)2)2 does not show any detectable cytotoxicity.",

keywords = "Cross cellular membrane, Fluorescence labeling, Fluorescence microscopy, Fullerene derivative C (C(COOH)), Real time imaging",

author = "Chang Ye and Chunying Chen and Zhen Chen and Huan Meng and Li Xing and Yaxin Jiang and Hui Yuan and Gengmei Xing and Feng Zhao and Yuliang Zhao and Zhifang Chai and Xiaohong Fang and Dong Han and Long Chen and Chen Wang and Taotao Wei",

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doi = "10.1007/s11434-006-1060-1",

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AU - Ye, Chang

AU - Chen, Chunying

AU - Chen, Zhen

AU - Meng, Huan

AU - Xing, Li

AU - Jiang, Yaxin

AU - Yuan, Hui

AU - Xing, Gengmei

AU - Zhao, Feng

AU - Zhao, Yuliang

AU - Chai, Zhifang

AU - Fang, Xiaohong

AU - Han, Dong

AU - Chen, Long

AU - Wang, Chen

AU - Wei, Taotao

PY - 2006/5

Y1 - 2006/5

N2 - The interactions between nanoparticles and living cells were investigated by an imaging technique of fluorescence microscopy. For this purpose, the C 60 derivative C60(C(COOH)2)2, a therapeutic agent for degeneration diseases of central nervous system, was synthesized, purified and characterized. Its interaction with the living cell and penetration of the cellular membrane were in situ studied using the real time imaging technique, and its potential cytotoxicity was also examined by flow cytometry. The results indicate that C60(C(COOH)2) 2 can easily enter cells, and is mainly located in cytoplasm by fluorescein labeling. Furthermore, C60(C(COOH)2) 2 can carry the molecule that cannot cross cellular membranes into cells, because fluorescein compound itself cannot enter the cell or adhere to membrane. At concentrations ranging from 1 × 10-2 to 1 × 102 mg/L, C60(C(COOH)2)2 does not show any detectable cytotoxicity.

AB - The interactions between nanoparticles and living cells were investigated by an imaging technique of fluorescence microscopy. For this purpose, the C 60 derivative C60(C(COOH)2)2, a therapeutic agent for degeneration diseases of central nervous system, was synthesized, purified and characterized. Its interaction with the living cell and penetration of the cellular membrane were in situ studied using the real time imaging technique, and its potential cytotoxicity was also examined by flow cytometry. The results indicate that C60(C(COOH)2) 2 can easily enter cells, and is mainly located in cytoplasm by fluorescein labeling. Furthermore, C60(C(COOH)2) 2 can carry the molecule that cannot cross cellular membranes into cells, because fluorescein compound itself cannot enter the cell or adhere to membrane. At concentrations ranging from 1 × 10-2 to 1 × 102 mg/L, C60(C(COOH)2)2 does not show any detectable cytotoxicity.

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KW - Fluorescence microscopy

KW - Fullerene derivative C (C(COOH))

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