TY - JOUR
T1 - In situ observation of C60(C(COOH)2)2 interacting with living cells using fluorescence microscopy
AU - Ye, Chang
AU - Chen, Chunying
AU - Chen, Zhen
AU - Meng, Huan
AU - Xing, Li
AU - Jiang, Yaxin
AU - Yuan, Hui
AU - Xing, Gengmei
AU - Zhao, Feng
AU - Zhao, Yuliang
AU - Chai, Zhifang
AU - Fang, Xiaohong
AU - Han, Dong
AU - Chen, Long
AU - Wang, Chen
AU - Wei, Taotao
N1 - Funding Information:
Acknowledgements The authors thank Prof. Liu Yuanfang of Peking University for his valuable suggestions and discussions. This work was supported by the National Natural Science Foundation of China (Grant Nos. 10490180 and 90406024), the Outstanding Young Natural Scientist Foundation (Grant Nos. 10525524 and 20225516), the Ministry of Science and Technology (Grant No. 2005CB724703), and the Directionality Program of the Chinese Academy of Sciences (Grant No. KJCX-SW-H12).
Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2006/5
Y1 - 2006/5
N2 - The interactions between nanoparticles and living cells were investigated by an imaging technique of fluorescence microscopy. For this purpose, the C 60 derivative C60(C(COOH)2)2, a therapeutic agent for degeneration diseases of central nervous system, was synthesized, purified and characterized. Its interaction with the living cell and penetration of the cellular membrane were in situ studied using the real time imaging technique, and its potential cytotoxicity was also examined by flow cytometry. The results indicate that C60(C(COOH)2) 2 can easily enter cells, and is mainly located in cytoplasm by fluorescein labeling. Furthermore, C60(C(COOH)2) 2 can carry the molecule that cannot cross cellular membranes into cells, because fluorescein compound itself cannot enter the cell or adhere to membrane. At concentrations ranging from 1 × 10-2 to 1 × 102 mg/L, C60(C(COOH)2)2 does not show any detectable cytotoxicity.
AB - The interactions between nanoparticles and living cells were investigated by an imaging technique of fluorescence microscopy. For this purpose, the C 60 derivative C60(C(COOH)2)2, a therapeutic agent for degeneration diseases of central nervous system, was synthesized, purified and characterized. Its interaction with the living cell and penetration of the cellular membrane were in situ studied using the real time imaging technique, and its potential cytotoxicity was also examined by flow cytometry. The results indicate that C60(C(COOH)2) 2 can easily enter cells, and is mainly located in cytoplasm by fluorescein labeling. Furthermore, C60(C(COOH)2) 2 can carry the molecule that cannot cross cellular membranes into cells, because fluorescein compound itself cannot enter the cell or adhere to membrane. At concentrations ranging from 1 × 10-2 to 1 × 102 mg/L, C60(C(COOH)2)2 does not show any detectable cytotoxicity.
KW - Cross cellular membrane
KW - Fluorescence labeling
KW - Fluorescence microscopy
KW - Fullerene derivative C (C(COOH))
KW - Real time imaging
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U2 - 10.1007/s11434-006-1060-1
DO - 10.1007/s11434-006-1060-1
M3 - Article
AN - SCOPUS:33748919846
VL - 51
SP - 1060
EP - 1064
JO - Chinese Science Bulletin
JF - Chinese Science Bulletin
SN - 1001-6538
IS - 9
ER -