In situ localization and chromosomal mapping of the AG1 (Dmp1) gene

A. George, J. Gui, N. A. Jenkins, D. J. Gilbert, N. G. Copeland, A. Veis

Research output: Contribution to journalArticlepeer-review

82 Scopus citations


Dentinogenesis is being used as a model for understanding the biomineralization process. The odontoblasts synthesize a structural matrix comprised of Type 1 collagen fibrils which define the basic architecture of the tissue. The odontoblasts also synthesize and deliver a number of dentin- specific acidic macromolecules into the extracellular compartment. These acidic macromolecules may be involved in regulating the ordered deposition of hydroxyapatite crystals within the matrix. AG1 is the first tooth-specific acidic macromolecule to have been cloned and sequenced. To identify which cells of the rat incisor pulp/odontoblast complex were responsible for synthesis of AG1, in situ hybridization was used. Digoxigenin labeled sense and anti-sense AG1 riboprobes were prepared. The AG1 mRNA was found to be expressed in the mature secretory odontoblasts. Neither pulp cells nor preodontoblasts showed any staining with the anti-sense probes. Chromosomal localization studies placed the AG1 gene on mouse chromosome 5q21, in tight linkage with Fgf5. AG1 has been renamed Dmp1 (dentin matrix protein 1) in accordance with present chromosomal nomenclature. Mouse 5q21 corresponds to the 4q21 locus in humans. This is the locus for the human tooth mineralization disorder dentinogenesis imperfecta Type II (DI-II). These data suggest that the Dmp1 gene is involved in mineralization and is a candidate gene for DI-II.

Original languageEnglish (US)
Pages (from-to)1527-1531
Number of pages5
JournalJournal of Histochemistry and Cytochemistry
Issue number12
StatePublished - 1994


  • AG1
  • Chromosomal localization
  • Dentin
  • Dentinogenesis imperfecta
  • Mineralization
  • Phosphoproteins

ASJC Scopus subject areas

  • Cell Biology
  • Anatomy


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