In search of suitable extraction technique for large scale commercial production of bioactive fraction for the treatment of diabetes: The case Diospyros melanoxylon Roxb

Md Harun Al Rashid, Sayani Majumder, Vivekananda Mandal, Subhash C. Mandal, Rajarajan Amirthalingam Thandavarayan

Research output: Contribution to journalArticle

Abstract

Diospyros melanoxylon Roxb. (D. melanoxylon) belongs to the family Ebenaceae and its leaves are very well known for making beedi throughout the World. The current study estimated the comparative extraction technique and its in-vitro antidiabetic prospective of the leaves of D. melanoxylon. Qualitative phytochemicals analysis of the samples from D. melanoxylon was carried out for the detection of secondary metabolites. Total phenolics, flavonoids, triterpenoids and tannins content of D. melanoxylon were estimated using colorimetric assay. Microwave-assisted extraction (MAE) technique with a low carbon output was observed for the speedy extraction of bioactive compounds obtained from Diospyros melanoxylon leaf extract. MAE produced a maximum yield of bioactive compounds which was found to be more efficient than ultrasound, soxhlet and maceration extraction. Qualitative HPLC analysis was performed for bioactive compounds. The in-vitro antidiabetic assay was performed using α-amylase and α-glucosidase inhibitory activity. In conclusion, the fractions exhibited the concentration-dependent inhibitory effect with significant (P < 0.0001) result. So the above performance might be accountable for the antidiabetic activity of D. Melanoxylon leaf extract due to presence of bioactive compounds.

Original languageEnglish (US)
Pages (from-to)106-118
Number of pages13
JournalJournal of Traditional and Complementary Medicine
Volume9
Issue number2
DOIs
StatePublished - Apr 2019

Keywords

  • Antidiabetic
  • D. melanoxylon
  • Extraction techniques
  • HPLC
  • Polyphenolics

ASJC Scopus subject areas

  • Complementary and alternative medicine

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