TY - JOUR
T1 - Improving the efficiency of RNA interference in mammals
AU - Mittal, Vivek
N1 - Funding Information:
The author thanks the members of his laboratory for critical reading of the manuscript and several other investigators for sharing their unpublished work. We acknowledge that space limitations might have precluded the citation of work of some investigators. The author was supported by grants from Cold Spring Harbor Laboratory and the National Institute of Health.
Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2004/5
Y1 - 2004/5
N2 - RNA interference (RNAi) has been very successfully applied as a gene-silencing technology in both plants and invertebrates, but many practical obstacles need to be overcome before it becomes viable in mammalian systems. Greater specificity and efficiency of RNAi in mammals is being achieved by improving the design and selection of small interfering RNAs (siRNAs), by increasing the efficacy of their delivery to cells and organisms, and by engineering their conditional expression. Genome-wide functional RNAi screens, which are predominantly done in worms and flies, have now begun to revolutionize large-scale loss-of-function studies in mammals.
AB - RNA interference (RNAi) has been very successfully applied as a gene-silencing technology in both plants and invertebrates, but many practical obstacles need to be overcome before it becomes viable in mammalian systems. Greater specificity and efficiency of RNAi in mammals is being achieved by improving the design and selection of small interfering RNAs (siRNAs), by increasing the efficacy of their delivery to cells and organisms, and by engineering their conditional expression. Genome-wide functional RNAi screens, which are predominantly done in worms and flies, have now begun to revolutionize large-scale loss-of-function studies in mammals.
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U2 - 10.1038/nrg1323
DO - 10.1038/nrg1323
M3 - Review article
C2 - 15143318
AN - SCOPUS:1942499451
SN - 1471-0056
VL - 5
SP - 355
EP - 365
JO - Nature Reviews Genetics
JF - Nature Reviews Genetics
IS - 5
ER -