Improving aspergillus niger tannase yield by N++ ion beam implantation

Wei Jin, Guangjun Nie, Hui Liu, Yang Xiaoran, Guohong Gong, Li Wang, Zhiming Zheng

Research output: Contribution to journalArticle

1 Scopus citations


This work aimed to improve tannase yield of Aspergillus niger through N+ion beam implantation in submerged fermentation. The energy and dose of N+ion beam implantation were investigated. The results indicated that an excellent mutant was obtained through nine successive implantations under the conditions of 10 keV and 30-40 (×2.6×1013) ions/cm2, and its tannase yield reached 38.5 U/mL, which was about five-time higher than the original strain. The study on the genetic stability of the mutant showed that its promising performance in tannase production could be stable. The studies of metal ions and surfactants affecting tannase yield indicated that manganese ions, stannum ions, xylene and SDS contained in the culture medium had positive effects on tannase production under submerged fermentation. Magnesium ions, in particular, could enhance the tannase yield by the mutant increasing by 42%, i.e. 53.6 U/mL. Accordingly, low-energy ion implantation could be a desirable approach to improve the fungal tannase yield for its commercial application.

Original languageEnglish (US)
Pages (from-to)135-142
Number of pages8
JournalBrazilian Archives of Biology and Technology
Issue number1
StatePublished - Jan 2013


  • Aspergillus niger
  • N ion implantation
  • Tannase

ASJC Scopus subject areas

  • General

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