Improved, shorter-latency carcinogen-induced hepatocellular carcinoma model in pigs

Jason Ho; Matthew Ware; Justin Law; Aaditya Nagaraj; Shilpa Jain; Jesse Rios; Reynaldo Calderon; Barry Toombs; Andrew Anderson; Collin Bray; Steven Curley; Stuart James Corr

doi:10.1159/000491092

Improved, shorter-latency carcinogen-induced hepatocellular carcinoma model in pigs

Jason Ho, Matthew Ware, Justin Law, Aaditya Nagaraj, Shilpa Jain, Jesse Rios, Reynaldo Calderon, Barry Toombs, Andrew Anderson, Collin Bray, Steven Curley, Stuart James Corr

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

Large animal models are important tools for hepatocellular carcinoma (HCC) research, especially in studies of hepatic vasculature, interventional techniques, and radiofrequency or microwave hyperthermia. Currently, diethylnitrosamine (DENA)-induced HCC in pigs is the only large animal model for in situ HCC with a tumor latency of 10-26 months. While phenobarbital (PB) is often used to accelerate DENA-induced HCC in rodents, it has not been previously studied in the porcine model. Therefore, we hypothesize that the addition of PB in the DENA-induced HCC porcine model will accelerate tumor latency compared to DENA alone. HCC and benign lesions were seen on serial MRI and confirmed on histopathology. Liver and tumors were further characterized by CT angiography, vascular corrosion casting, and permittivity measurements.

Original language	English (US)
Pages (from-to)	360-369
Number of pages	10
Journal	Oncology (Switzerland)
Volume	95
Issue number	6
DOIs	https://doi.org/10.1159/000491092
State	Published - Nov 1 2018

Keywords

Diethylnitrosamine
Hepatocellular carcinoma
Phenobarbital
Porcine

ASJC Scopus subject areas

Oncology
Cancer Research

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10.1159/000491092

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title = "Improved, shorter-latency carcinogen-induced hepatocellular carcinoma model in pigs",

abstract = "Large animal models are important tools for hepatocellular carcinoma (HCC) research, especially in studies of hepatic vasculature, interventional techniques, and radiofrequency or microwave hyperthermia. Currently, diethylnitrosamine (DENA)-induced HCC in pigs is the only large animal model for in situ HCC with a tumor latency of 10-26 months. While phenobarbital (PB) is often used to accelerate DENA-induced HCC in rodents, it has not been previously studied in the porcine model. Therefore, we hypothesize that the addition of PB in the DENA-induced HCC porcine model will accelerate tumor latency compared to DENA alone. HCC and benign lesions were seen on serial MRI and confirmed on histopathology. Liver and tumors were further characterized by CT angiography, vascular corrosion casting, and permittivity measurements.",

keywords = "Diethylnitrosamine, Hepatocellular carcinoma, Phenobarbital, Porcine",

author = "Jason Ho and Matthew Ware and Justin Law and Aaditya Nagaraj and Shilpa Jain and Jesse Rios and Reynaldo Calderon and Barry Toombs and Andrew Anderson and Collin Bray and Steven Curley and Corr, {Stuart James}",

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doi = "10.1159/000491092",

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T1 - Improved, shorter-latency carcinogen-induced hepatocellular carcinoma model in pigs

AU - Ho, Jason

AU - Ware, Matthew

AU - Law, Justin

AU - Nagaraj, Aaditya

AU - Jain, Shilpa

AU - Rios, Jesse

AU - Calderon, Reynaldo

AU - Toombs, Barry

AU - Anderson, Andrew

AU - Bray, Collin

AU - Curley, Steven

AU - Corr, Stuart James

PY - 2018/11/1

Y1 - 2018/11/1

N2 - Large animal models are important tools for hepatocellular carcinoma (HCC) research, especially in studies of hepatic vasculature, interventional techniques, and radiofrequency or microwave hyperthermia. Currently, diethylnitrosamine (DENA)-induced HCC in pigs is the only large animal model for in situ HCC with a tumor latency of 10-26 months. While phenobarbital (PB) is often used to accelerate DENA-induced HCC in rodents, it has not been previously studied in the porcine model. Therefore, we hypothesize that the addition of PB in the DENA-induced HCC porcine model will accelerate tumor latency compared to DENA alone. HCC and benign lesions were seen on serial MRI and confirmed on histopathology. Liver and tumors were further characterized by CT angiography, vascular corrosion casting, and permittivity measurements.

AB - Large animal models are important tools for hepatocellular carcinoma (HCC) research, especially in studies of hepatic vasculature, interventional techniques, and radiofrequency or microwave hyperthermia. Currently, diethylnitrosamine (DENA)-induced HCC in pigs is the only large animal model for in situ HCC with a tumor latency of 10-26 months. While phenobarbital (PB) is often used to accelerate DENA-induced HCC in rodents, it has not been previously studied in the porcine model. Therefore, we hypothesize that the addition of PB in the DENA-induced HCC porcine model will accelerate tumor latency compared to DENA alone. HCC and benign lesions were seen on serial MRI and confirmed on histopathology. Liver and tumors were further characterized by CT angiography, vascular corrosion casting, and permittivity measurements.

KW - Diethylnitrosamine

KW - Hepatocellular carcinoma

KW - Phenobarbital

KW - Porcine

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JO - Oncology (Switzerland)

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ER -

Improved, shorter-latency carcinogen-induced hepatocellular carcinoma model in pigs

Abstract

Keywords

ASJC Scopus subject areas

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