Improved detection of Bartonella DNA in mammalian hosts and arthropod vectors by real-time PCR using the NADH dehydrogenase gamma subunit (nuoG)

James M. Colborn; Michael Y. Kosoy; Vladimir L. Motin; Maxim V. Telepnev; Gustavo Valbuena; Khin S. Myint; Yuri Fofanov; Catherine Putonti; Chen Feng; Leonard Peruski

doi:10.1128/JCM.00470-10

Improved detection of Bartonella DNA in mammalian hosts and arthropod vectors by real-time PCR using the NADH dehydrogenase gamma subunit (nuoG)

James M. Colborn, Michael Y. Kosoy, Vladimir L. Motin, Maxim V. Telepnev, Gustavo Valbuena, Khin S. Myint, Yuri Fofanov, Catherine Putonti, Chen Feng, Leonard Peruski

Houston Methodist

Research output: Contribution to journal › Article › peer-review

55 Scopus citations

Abstract

We used a whole-genome scanning technique to identify the NADH dehydrogenase gamma subunit (nuoG) primer set that is sensitive and specific enough to detect a diverse number of Bartonella species in a wide range of environmental samples yet maintains minimal cross-reactivity to mammalian host and arthropod vector organisms.

Original language	English (US)
Pages (from-to)	4630-4633
Number of pages	4
Journal	Journal of Clinical Microbiology
Volume	48
Issue number	12
DOIs	https://doi.org/10.1128/JCM.00470-10
State	Published - Dec 2010

ASJC Scopus subject areas

Microbiology (medical)

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Colborn, J. M., Kosoy, M. Y., Motin, V. L., Telepnev, M. V., Valbuena, G., Myint, K. S., Fofanov, Y., Putonti, C., Feng, C., & Peruski, L. (2010). Improved detection of Bartonella DNA in mammalian hosts and arthropod vectors by real-time PCR using the NADH dehydrogenase gamma subunit (nuoG). Journal of Clinical Microbiology, 48(12), 4630-4633. https://doi.org/10.1128/JCM.00470-10

Colborn, JM, Kosoy, MY, Motin, VL, Telepnev, MV, Valbuena, G, Myint, KS, Fofanov, Y, Putonti, C, Feng, C & Peruski, L 2010, 'Improved detection of Bartonella DNA in mammalian hosts and arthropod vectors by real-time PCR using the NADH dehydrogenase gamma subunit (nuoG)', Journal of Clinical Microbiology, vol. 48, no. 12, pp. 4630-4633. https://doi.org/10.1128/JCM.00470-10

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AU - Motin, Vladimir L.

AU - Telepnev, Maxim V.

AU - Valbuena, Gustavo

AU - Myint, Khin S.

AU - Fofanov, Yuri

AU - Putonti, Catherine

AU - Feng, Chen

AU - Peruski, Leonard

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Improved detection of Bartonella DNA in mammalian hosts and arthropod vectors by real-time PCR using the NADH dehydrogenase gamma subunit (nuoG)

Abstract

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