Improved cardiovascular function in old mice after N-Acetyl cysteine and glycine supplemented diet: Inflammation and mitochondrial factors

Research output: Contribution to journalArticle

Katarzyna A. Cieslik, Rajagopal V. Sekhar, Alejandro Granillo, Anilkumar Reddy, Guillermo Medrano, Celia Pena Heredia, Mark L. Entman, Dale J. Hamilton, Shumin Li, Erin L. Reineke, Anisha A. Gupte, Aijun Zhang, George Taffet

Metabolic, inflammatory, and functional changes occur in cardiovascular aging which may stem from oxidative stress and be remediable with antioxidants. Glutathione, an intracellular antioxidant, declines with aging, and supplementation with glutathione precursors, N-acetyl cysteine (NAC) and glycine (Gly), increases tissue glutathione. Thirty-month old mice were fed diets supplemented with NAC or NAC+Gly and, after 7 weeks, cardiac function and molecular studies were performed. The NAC+Gly supplementation improved diastolic function, increasing peak early filling velocity, and reducing relaxation time, left atrial volume, and left ventricle end diastolic pressure. By contrast, cardiac function did not improve with NAC alone. Both diet supplementations decreased cardiac levels of inflammatory mediators; only NAC+Gly reduced leukocyte infiltration. Several mitochondrial genes reduced with aging were upregulated in hearts by NAC+Gly diet supplementation. These Krebs cycle and oxidative phosphorylation enzymes, suggesting improved mitochondrial function, and permeabilized cardiac fibers from NAC+Gly-fed mice produced ATP from carbohydrate and fatty acid sources, whereas fibers from control old mice were less able to utilize fatty acids. Our data indicate that NAC+Gly supplementation can improve diastolic function in the old mouse and may have potential to prevent important morbidities for older people.

Original languageEnglish (US)
Pages (from-to)1167-1177
Number of pages11
JournalJournals of Gerontology - Series A Biological Sciences and Medical Sciences
Volume73
Issue number9
DOIs
StatePublished - Jan 1 2018

PMID: 29538624

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Improved cardiovascular function in old mice after N-Acetyl cysteine and glycine supplemented diet : Inflammation and mitochondrial factors. / Cieslik, Katarzyna A.; Sekhar, Rajagopal V.; Granillo, Alejandro; Reddy, Anilkumar; Medrano, Guillermo; Heredia, Celia Pena; Entman, Mark L.; Hamilton, Dale J.; Li, Shumin; Reineke, Erin L.; Gupte, Anisha A.; Zhang, Aijun; Taffet, George.

In: Journals of Gerontology - Series A Biological Sciences and Medical Sciences, Vol. 73, No. 9, 01.01.2018, p. 1167-1177.

Research output: Contribution to journalArticle

Harvard

Cieslik, KA, Sekhar, RV, Granillo, A, Reddy, A, Medrano, G, Heredia, CP, Entman, ML, Hamilton, DJ, Li, S, Reineke, EL, Gupte, AA, Zhang, A & Taffet, G 2018, 'Improved cardiovascular function in old mice after N-Acetyl cysteine and glycine supplemented diet: Inflammation and mitochondrial factors' Journals of Gerontology - Series A Biological Sciences and Medical Sciences, vol. 73, no. 9, pp. 1167-1177. https://doi.org/10.1093/gerona/gly034

APA

Cieslik, K. A., Sekhar, R. V., Granillo, A., Reddy, A., Medrano, G., Heredia, C. P., ... Taffet, G. (2018). Improved cardiovascular function in old mice after N-Acetyl cysteine and glycine supplemented diet: Inflammation and mitochondrial factors. Journals of Gerontology - Series A Biological Sciences and Medical Sciences, 73(9), 1167-1177. https://doi.org/10.1093/gerona/gly034

Vancouver

Cieslik KA, Sekhar RV, Granillo A, Reddy A, Medrano G, Heredia CP et al. Improved cardiovascular function in old mice after N-Acetyl cysteine and glycine supplemented diet: Inflammation and mitochondrial factors. Journals of Gerontology - Series A Biological Sciences and Medical Sciences. 2018 Jan 1;73(9):1167-1177. https://doi.org/10.1093/gerona/gly034

Author

Cieslik, Katarzyna A. ; Sekhar, Rajagopal V. ; Granillo, Alejandro ; Reddy, Anilkumar ; Medrano, Guillermo ; Heredia, Celia Pena ; Entman, Mark L. ; Hamilton, Dale J. ; Li, Shumin ; Reineke, Erin L. ; Gupte, Anisha A. ; Zhang, Aijun ; Taffet, George. / Improved cardiovascular function in old mice after N-Acetyl cysteine and glycine supplemented diet : Inflammation and mitochondrial factors. In: Journals of Gerontology - Series A Biological Sciences and Medical Sciences. 2018 ; Vol. 73, No. 9. pp. 1167-1177.

BibTeX

@article{8258947b3a64414ea16bc052696723df,
title = "Improved cardiovascular function in old mice after N-Acetyl cysteine and glycine supplemented diet: Inflammation and mitochondrial factors",
abstract = "Metabolic, inflammatory, and functional changes occur in cardiovascular aging which may stem from oxidative stress and be remediable with antioxidants. Glutathione, an intracellular antioxidant, declines with aging, and supplementation with glutathione precursors, N-acetyl cysteine (NAC) and glycine (Gly), increases tissue glutathione. Thirty-month old mice were fed diets supplemented with NAC or NAC+Gly and, after 7 weeks, cardiac function and molecular studies were performed. The NAC+Gly supplementation improved diastolic function, increasing peak early filling velocity, and reducing relaxation time, left atrial volume, and left ventricle end diastolic pressure. By contrast, cardiac function did not improve with NAC alone. Both diet supplementations decreased cardiac levels of inflammatory mediators; only NAC+Gly reduced leukocyte infiltration. Several mitochondrial genes reduced with aging were upregulated in hearts by NAC+Gly diet supplementation. These Krebs cycle and oxidative phosphorylation enzymes, suggesting improved mitochondrial function, and permeabilized cardiac fibers from NAC+Gly-fed mice produced ATP from carbohydrate and fatty acid sources, whereas fibers from control old mice were less able to utilize fatty acids. Our data indicate that NAC+Gly supplementation can improve diastolic function in the old mouse and may have potential to prevent important morbidities for older people.",
keywords = "Diastolic dysfunction, Inflammation, Mitochondria",
author = "Cieslik, {Katarzyna A.} and Sekhar, {Rajagopal V.} and Alejandro Granillo and Anilkumar Reddy and Guillermo Medrano and Heredia, {Celia Pena} and Entman, {Mark L.} and Hamilton, {Dale J.} and Shumin Li and Reineke, {Erin L.} and Gupte, {Anisha A.} and Aijun Zhang and George Taffet",
year = "2018",
month = "1",
day = "1",
doi = "10.1093/gerona/gly034",
language = "English (US)",
volume = "73",
pages = "1167--1177",
journal = "Journals of Gerontology - Series A Biological Sciences and Medical Sciences",
issn = "1079-5006",
publisher = "Oxford University Press",
number = "9",

}

RIS

TY - JOUR

T1 - Improved cardiovascular function in old mice after N-Acetyl cysteine and glycine supplemented diet

T2 - Journals of Gerontology - Series A Biological Sciences and Medical Sciences

AU - Cieslik, Katarzyna A.

AU - Sekhar, Rajagopal V.

AU - Granillo, Alejandro

AU - Reddy, Anilkumar

AU - Medrano, Guillermo

AU - Heredia, Celia Pena

AU - Entman, Mark L.

AU - Hamilton, Dale J.

AU - Li, Shumin

AU - Reineke, Erin L.

AU - Gupte, Anisha A.

AU - Zhang, Aijun

AU - Taffet, George

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Metabolic, inflammatory, and functional changes occur in cardiovascular aging which may stem from oxidative stress and be remediable with antioxidants. Glutathione, an intracellular antioxidant, declines with aging, and supplementation with glutathione precursors, N-acetyl cysteine (NAC) and glycine (Gly), increases tissue glutathione. Thirty-month old mice were fed diets supplemented with NAC or NAC+Gly and, after 7 weeks, cardiac function and molecular studies were performed. The NAC+Gly supplementation improved diastolic function, increasing peak early filling velocity, and reducing relaxation time, left atrial volume, and left ventricle end diastolic pressure. By contrast, cardiac function did not improve with NAC alone. Both diet supplementations decreased cardiac levels of inflammatory mediators; only NAC+Gly reduced leukocyte infiltration. Several mitochondrial genes reduced with aging were upregulated in hearts by NAC+Gly diet supplementation. These Krebs cycle and oxidative phosphorylation enzymes, suggesting improved mitochondrial function, and permeabilized cardiac fibers from NAC+Gly-fed mice produced ATP from carbohydrate and fatty acid sources, whereas fibers from control old mice were less able to utilize fatty acids. Our data indicate that NAC+Gly supplementation can improve diastolic function in the old mouse and may have potential to prevent important morbidities for older people.

AB - Metabolic, inflammatory, and functional changes occur in cardiovascular aging which may stem from oxidative stress and be remediable with antioxidants. Glutathione, an intracellular antioxidant, declines with aging, and supplementation with glutathione precursors, N-acetyl cysteine (NAC) and glycine (Gly), increases tissue glutathione. Thirty-month old mice were fed diets supplemented with NAC or NAC+Gly and, after 7 weeks, cardiac function and molecular studies were performed. The NAC+Gly supplementation improved diastolic function, increasing peak early filling velocity, and reducing relaxation time, left atrial volume, and left ventricle end diastolic pressure. By contrast, cardiac function did not improve with NAC alone. Both diet supplementations decreased cardiac levels of inflammatory mediators; only NAC+Gly reduced leukocyte infiltration. Several mitochondrial genes reduced with aging were upregulated in hearts by NAC+Gly diet supplementation. These Krebs cycle and oxidative phosphorylation enzymes, suggesting improved mitochondrial function, and permeabilized cardiac fibers from NAC+Gly-fed mice produced ATP from carbohydrate and fatty acid sources, whereas fibers from control old mice were less able to utilize fatty acids. Our data indicate that NAC+Gly supplementation can improve diastolic function in the old mouse and may have potential to prevent important morbidities for older people.

KW - Diastolic dysfunction

KW - Inflammation

KW - Mitochondria

UR - http://www.scopus.com/inward/record.url?scp=85055569885&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85055569885&partnerID=8YFLogxK

U2 - 10.1093/gerona/gly034

DO - 10.1093/gerona/gly034

M3 - Article

VL - 73

SP - 1167

EP - 1177

JO - Journals of Gerontology - Series A Biological Sciences and Medical Sciences

JF - Journals of Gerontology - Series A Biological Sciences and Medical Sciences

SN - 1079-5006

IS - 9

ER -

Similar Research Outputs

  1. Improved Cardiovascular Function in Old Mice After N-Acetyl Cysteine and Glycine Supplemented Diet: Inflammation and Mitochondrial Factors

    Cieslik, K. A., Sekhar, R. V., Granillo, A., Reddy, A., Medrano, G., Heredia, C. P., Entman, M. L., Hamilton, D. J., Li, S., Reineke, E., Gupte, A. A., Zhang, A. & Taffet, G. E., Aug 10 2018, In : The journals of gerontology. Series A, Biological sciences and medical sciences. 73, 9, p. 1167-1177 11 p.

    Research output: Contribution to journalArticle

ID: 42295975