TY - JOUR
T1 - Improved auxanographic method for yeast assimilations
T2 - a comparison with other approaches
AU - Land, Geoffrey
AU - Vinton, E. C.
AU - Adcock, G. B.
AU - Hopkins, J. M.
PY - 1975/12/1
Y1 - 1975/12/1
N2 - An improved pour plate auxanographic method has been developed for determining the assimilation of 14 different carbohydrates by medically important yeasts. Reduction of a dye incorporated into the agar has been correlated with the growth and carbohydrate assimilation of the yeasts, allowing the speciation of many yeasts within 24 to 48 h. This technique has been found to compare more than favorably with existing yeast assimilation techniques, in terms of rapid identification, total cost, and technician time in preparing and inoculating the plates. The dye pour plate auxanographic technique provides an easier to interpret, rapid, and reproducible method of mycological identification for small clinical laboratories.
AB - An improved pour plate auxanographic method has been developed for determining the assimilation of 14 different carbohydrates by medically important yeasts. Reduction of a dye incorporated into the agar has been correlated with the growth and carbohydrate assimilation of the yeasts, allowing the speciation of many yeasts within 24 to 48 h. This technique has been found to compare more than favorably with existing yeast assimilation techniques, in terms of rapid identification, total cost, and technician time in preparing and inoculating the plates. The dye pour plate auxanographic technique provides an easier to interpret, rapid, and reproducible method of mycological identification for small clinical laboratories.
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M3 - Article
C2 - 1176628
AN - SCOPUS:0016736241
VL - 2
SP - 206
EP - 217
JO - Journal of Clinical Microbiology
JF - Journal of Clinical Microbiology
SN - 0095-1137
IS - 3
ER -