Importance of two Enterococcus faecium loci encoding Gls-like proteins for in vitro bile salts stress response and virulence

Tina Choudhury, Kavindra V. Singh, Jouko Sillanpää, Sreedhar R. Nallapareddy, Barbara E. Murray

Research output: Contribution to journalArticlepeer-review

Abstract

General stress proteins, Gls24 and GlsB, were previously shown to be involved in bile salts resistance of Enterococcus faecalis and in virulence. Here, we identified 2 gene clusters in Enterococcus faecium each encoding a homolog of Gls24 (Gls33 and Gls20; designated on the basis of their predicted sizes) and of GlsB (GlsB and GlsB1). The sequences of the gls33 and gls20 gene clusters from available genomes indicate distinct lineages, with those of hospital-associated CC17 isolates differing from non-CC17 by ∼7% and ∼3.5%, respectively. Deletion of an individual locus did not have a significant effect on virulence in a mouse peritonitis model, whereas a double-deletion mutant was highly attenuated (P<.004) versus wild-type. However, mutants lacking either gls33-glsB, gls20-glsB1, or both all exhibited increased sensitivity to bile salts. These results suggest that gls-encoded loci may be important for adaptation to the intestinal environment, in addition to being important for virulence functions.

Original languageEnglish (US)
Pages (from-to)1147-1154
Number of pages8
JournalJournal of Infectious Diseases
Volume203
Issue number8
DOIs
StatePublished - Apr 15 2011

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

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