Importance of the epa locus of enterococcus faecalis OGlRF in a mouse model of ascending urinary tract infection

Kavindra V. Singh; Roshan J. Lewis; Barbara E. Murray

doi:10.1086/600124

Importance of the epa locus of enterococcus faecalis OGlRF in a mouse model of ascending urinary tract infection

Kavindra V. Singh, Roshan J. Lewis, Barbara E. Murray

Research output: Contribution to journal › Article › peer-review

31 Scopus citations

Abstract

Previously, TX5179, a disruption mutant of the enterococcal polysaccharide antigen (epa) gene cluster of Enterococcusfaecalis strain OGlRF was shown to be attenuated in translocation, biofilm, mouse peritonitis and was more susceptible to polymorphonuclear leukocyte phagocytic killing. Here, wild-type E. faecalis OGlRF and TX5179 strains were tested in a mixed-infection (inoculum, -1:1) mouse urinary tract infection model. Wild-type OGlRF outnumbered TX5179 in the kidneys (P < .001) and bladder (P < .001). In conclusion, the epa locus of E. faecalis OGlRF contributes to murine urinary tract infection and is the first such enterococcal polysaccharide locus shown to be important in this site.

Original language	English (US)
Pages (from-to)	417-420
Number of pages	4
Journal	Journal of Infectious Diseases
Volume	200
Issue number	3
DOIs	https://doi.org/10.1086/600124
State	Published - Aug 1 2009

ASJC Scopus subject areas

Immunology and Allergy
Infectious Diseases

Access to Document

10.1086/600124

Cite this

@article{96bf4ed212ae479d937ef7a42768ceac,

title = "Importance of the epa locus of enterococcus faecalis OGlRF in a mouse model of ascending urinary tract infection",

abstract = "Previously, TX5179, a disruption mutant of the enterococcal polysaccharide antigen (epa) gene cluster of Enterococcusfaecalis strain OGlRF was shown to be attenuated in translocation, biofilm, mouse peritonitis and was more susceptible to polymorphonuclear leukocyte phagocytic killing. Here, wild-type E. faecalis OGlRF and TX5179 strains were tested in a mixed-infection (inoculum, -1:1) mouse urinary tract infection model. Wild-type OGlRF outnumbered TX5179 in the kidneys (P < .001) and bladder (P < .001). In conclusion, the epa locus of E. faecalis OGlRF contributes to murine urinary tract infection and is the first such enterococcal polysaccharide locus shown to be important in this site.",

author = "Singh, {Kavindra V.} and Lewis, {Roshan J.} and Murray, {Barbara E.}",

year = "2009",

month = aug,

day = "1",

doi = "10.1086/600124",

language = "English (US)",

volume = "200",

pages = "417--420",

journal = "Journal of Infectious Diseases",

issn = "0022-1899",

publisher = "Oxford University Press",

number = "3",

}

TY - JOUR

T1 - Importance of the epa locus of enterococcus faecalis OGlRF in a mouse model of ascending urinary tract infection

AU - Singh, Kavindra V.

AU - Lewis, Roshan J.

AU - Murray, Barbara E.

PY - 2009/8/1

Y1 - 2009/8/1

N2 - Previously, TX5179, a disruption mutant of the enterococcal polysaccharide antigen (epa) gene cluster of Enterococcusfaecalis strain OGlRF was shown to be attenuated in translocation, biofilm, mouse peritonitis and was more susceptible to polymorphonuclear leukocyte phagocytic killing. Here, wild-type E. faecalis OGlRF and TX5179 strains were tested in a mixed-infection (inoculum, -1:1) mouse urinary tract infection model. Wild-type OGlRF outnumbered TX5179 in the kidneys (P < .001) and bladder (P < .001). In conclusion, the epa locus of E. faecalis OGlRF contributes to murine urinary tract infection and is the first such enterococcal polysaccharide locus shown to be important in this site.

AB - Previously, TX5179, a disruption mutant of the enterococcal polysaccharide antigen (epa) gene cluster of Enterococcusfaecalis strain OGlRF was shown to be attenuated in translocation, biofilm, mouse peritonitis and was more susceptible to polymorphonuclear leukocyte phagocytic killing. Here, wild-type E. faecalis OGlRF and TX5179 strains were tested in a mixed-infection (inoculum, -1:1) mouse urinary tract infection model. Wild-type OGlRF outnumbered TX5179 in the kidneys (P < .001) and bladder (P < .001). In conclusion, the epa locus of E. faecalis OGlRF contributes to murine urinary tract infection and is the first such enterococcal polysaccharide locus shown to be important in this site.

UR - http://www.scopus.com/inward/record.url?scp=67650667802&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=67650667802&partnerID=8YFLogxK

U2 - 10.1086/600124

DO - 10.1086/600124

M3 - Article

C2 - 19545208

AN - SCOPUS:67650667802

VL - 200

SP - 417

EP - 420

JO - Journal of Infectious Diseases

JF - Journal of Infectious Diseases

SN - 0022-1899

IS - 3

ER -

Importance of the epa locus of enterococcus faecalis OGlRF in a mouse model of ascending urinary tract infection

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this