Importance of the epa locus of enterococcus faecalis OGlRF in a mouse model of ascending urinary tract infection

Kavindra V. Singh; Roshan J. Lewis; Barbara E. Murray

doi:10.1086/600124

Importance of the epa locus of enterococcus faecalis OGlRF in a mouse model of ascending urinary tract infection

Kavindra V. Singh, Roshan J. Lewis, Barbara E. Murray

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34 Scopus citations

Abstract

Previously, TX5179, a disruption mutant of the enterococcal polysaccharide antigen (epa) gene cluster of Enterococcusfaecalis strain OGlRF was shown to be attenuated in translocation, biofilm, mouse peritonitis and was more susceptible to polymorphonuclear leukocyte phagocytic killing. Here, wild-type E. faecalis OGlRF and TX5179 strains were tested in a mixed-infection (inoculum, -1:1) mouse urinary tract infection model. Wild-type OGlRF outnumbered TX5179 in the kidneys (P < .001) and bladder (P < .001). In conclusion, the epa locus of E. faecalis OGlRF contributes to murine urinary tract infection and is the first such enterococcal polysaccharide locus shown to be important in this site.

Original language	English (US)
Pages (from-to)	417-420
Number of pages	4
Journal	Journal of Infectious Diseases
Volume	200
Issue number	3
DOIs	https://doi.org/10.1086/600124
State	Published - Aug 1 2009

ASJC Scopus subject areas

General Medicine

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title = "Importance of the epa locus of enterococcus faecalis OGlRF in a mouse model of ascending urinary tract infection",

abstract = "Previously, TX5179, a disruption mutant of the enterococcal polysaccharide antigen (epa) gene cluster of Enterococcusfaecalis strain OGlRF was shown to be attenuated in translocation, biofilm, mouse peritonitis and was more susceptible to polymorphonuclear leukocyte phagocytic killing. Here, wild-type E. faecalis OGlRF and TX5179 strains were tested in a mixed-infection (inoculum, -1:1) mouse urinary tract infection model. Wild-type OGlRF outnumbered TX5179 in the kidneys (P < .001) and bladder (P < .001). In conclusion, the epa locus of E. faecalis OGlRF contributes to murine urinary tract infection and is the first such enterococcal polysaccharide locus shown to be important in this site.",

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AB - Previously, TX5179, a disruption mutant of the enterococcal polysaccharide antigen (epa) gene cluster of Enterococcusfaecalis strain OGlRF was shown to be attenuated in translocation, biofilm, mouse peritonitis and was more susceptible to polymorphonuclear leukocyte phagocytic killing. Here, wild-type E. faecalis OGlRF and TX5179 strains were tested in a mixed-infection (inoculum, -1:1) mouse urinary tract infection model. Wild-type OGlRF outnumbered TX5179 in the kidneys (P < .001) and bladder (P < .001). In conclusion, the epa locus of E. faecalis OGlRF contributes to murine urinary tract infection and is the first such enterococcal polysaccharide locus shown to be important in this site.

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Importance of the epa locus of enterococcus faecalis OGlRF in a mouse model of ascending urinary tract infection

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