Implementation of a novel web-based lesion selection tool to improve acquisition of tumor biopsy specimens

Mingxuan Xu, Coya Tapia, Joud Hajjar, Sharjeel Sabir, Rivka Colen, Priyadharsini Nagarajan, Phyu P. Aung, Jing Gong, Jordi Rodon, Siqing Fu, Bettzy Stephen, Sinchita Roy-Chowdhuri, Hung Le, Vincent Yang, Abdulrazzak Zarifa, Mohamed Elsayed Abdelsalam, Anuja Jhingran, Milind Javle, Shubham Pant, Brett CarterDenai R. Milton, Ryan Sun, Daniel D. Karp, Eugene Jon Koay, Yali Yang, Ignacio I. Wistuba, Patrick Hwu, Funda Meric-Bernstam, Aung Naing

Research output: Contribution to journalArticlepeer-review

4 Scopus citations


Introduction: For maximum utility of molecular characterization by next-generation sequencing (NGS) and better understanding of tumor microenvironment with immune correlates analysis, biopsy specimens must yield adequate tumor tissue, and sequential biopsy specimens should sample a consistent site. We developed a web-based lesion selection tool (LST) that enables management and tracking of the biopsy specimen collections. Methods: Of 145 patients, the LST was used for 88 patients; the other 57 served as controls. We evaluated consistency of the lesion biopsied in longitudinal collections, number of cores obtained, and cores with adequate tumor cellularity for NGS. The Fisher exact test and Wilcoxon rank sum test were used to identify differences between the groups. Results: The analysis included 30 of 88 (34%) patients in the LST group and 52 of 57 (91%) in the control group. The LST workflow ensured 100% consistency in the lesions biopsied compared with 75% in the control group in longitudinal collections and increased the proportion of patients in whom at least five cores were collected per biopsy. Conclusions: The novel LST platform facilitates coordination, performance, and management of longitudinal biopsy specimens. Use of the LST enables sampling of the designated lesion consistently, which is likely to accurately inform us the effect of the treatment on tumor microenvironment and evolution of resistant pathways. Such studies are important translational component of any clinical trials and research as they guide the development of next line of therapy, which has significant effect on clinical utility. However, validation of this approach in a larger study is warranted.

Original languageEnglish (US)
Pages (from-to)45-52
Number of pages8
JournalJournal of Immunotherapy and Precision Oncology
Issue number2
StatePublished - 2021


  • Biomarker
  • Biopsy acquisition
  • Biopsy specimen
  • Clinical study
  • Lesion selection

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Oncology
  • Cancer Research


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