Immunostimulatory properties and enhanced TNF- α mediated cellular immunity for tumor therapy by C60(OH)20 nanoparticles

Ying Liu, Fang Jiao, Yang Qiu, Wei Li, Ying Qu, Chixia Tian, Yufeng Li, Ru Bai, Fang Lao, Yuliang Zhao, Zhifang Chai, Chunying Chen

Research output: Contribution to journalArticlepeer-review

89 Scopus citations

Abstract

Publications concerning the mechanism of biological activity, especially the immunological mechanism of C60(OH)20 nanoparticles, are relatively limited. However, the structure and characteristics of this carbon allotrope have been widely investigated. In this paper, we have demonstrated that water-soluble C60(OH)20 nanoparticles have an efficient anti-tumor activity in vivo, and show specific immunomodulatory effects to the immune cells, such as T cells and macrophages, both in vivo and in vitro. For example, C60(OH)20 nanoparticles can increase the production of T-helper cell type 1 (Th1) cytokines (IL-2, IFN- γ and TNF-α), and decrease the production of Th2 cytokines (IL-4, IL-5 and IL-6) in serum samples. On the other hand, C 60(OH)20 nanoparticles show almost no adverse effect to the viability of immune cells in vitro but stimulate the immune cells to release more cytokines, in particular TNF- α, which plays a key role in the cellular immune process to help eliminate abnormal cells. TNF- α production increased almost three-fold in treated T lymphocytes and macrophages. Accordingly, we conclude that C60(OH)20 nanoparticles have an efficient anti-tumor activity and this effect is associated with an increased CD4 +/CD8 + lymphocyte ratio and the enhancement of TNF- α production. The data suggest that C 60(OH)20 nanoparticles can improve the immune response to help to scavenge and kill tumor cells.

Original languageEnglish (US)
Article number415102
JournalNanotechnology
Volume20
Issue number41
DOIs
StatePublished - Oct 12 2009

ASJC Scopus subject areas

  • Bioengineering
  • Chemistry(all)
  • Materials Science(all)
  • Mechanics of Materials
  • Mechanical Engineering
  • Electrical and Electronic Engineering

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