Immunohistochemical localization of granzyme B antigen in cytotoxic cells in human tissues

A. Hameed, Luan Truong, V. Price, O. Kruhenbuhl, J. Tschopp

Research output: Contribution to journalArticlepeer-review

24 Scopus citations


Human granzyme B antigen is expressed in cytoplasmic granules of activated cytotoxic T lymphocytes and natural killer cells. Recombinant granzyme B was generated using aprokaryotic expression vector under the control of T 7 transcription and translation signals. The 25-kd recombinant protein (granzyme B) was used to develop a rabbit polyclonal antiserum. Purified anti-granzyme B antibodies were used to detect the antigen expression in cytotoxic cells in human tissues. Using the avidin-biotin-complex peroxidase technique, formalin-fixed, paraffin-embedded tissue sections from patients with acute mild or moderate allograft cardiac rejection were stained. A constant cytoplasmic staining of the lymphocytic allograft infiltrate was observed. These results provide a basis for using the anti-granzyme B antibodies for detection of cytotoxic cells in human tissues. The detection and quantitative analysis of the granzyme-associated cytotoxic cells may help to evaluate the significance of these functionally distinct cytotoxic cells in human tissues associated with increased expression of cytoplasmic granule effector molecules.

Original languageEnglish (US)
Pages (from-to)1069-1075
Number of pages7
JournalAmerican Journal of Pathology
Issue number5
StatePublished - Jan 1 1991

ASJC Scopus subject areas

  • Pathology and Forensic Medicine


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