Immunohistochemical detection of hepatocellular carcinoma in the setting of ongoing necrosis after radiofrequency ablation

Tomoo Itoh, Yasuko Orba, Hidehiro Takei, Yusuke Ishida, Makoto Saitoh, Hideaki Nakamura, Takashi Meguro, Shoichi Horita, Miri Fujita, Kazuo Nagashima

Research output: Contribution to journalArticle

33 Scopus citations

Abstract

After radiofrequency ablation (RFA), hepatocellular carcinoma undergoes complete necrosis and an ongoing necrosis that is irreversible and characterized histologically by disrupted cell outlines, homogenous cytoplasmic eosinophilia, and preserved nuclear staining, with the cells appearing quite distinct from viable cancer cells. Antibody to detect single-stranded DNA (ssDNA) specifically labeled nuclei in the setting of ongoing necrosis, but not viable tumor cells, whereas human mitochondrial antibody labeled the cytoplasm of viable cells but not cells of ongoing necrosis. The results demonstrate that RFA causes denaturation of both DNA and proteins and that the immunohistochemistry of ssDNA and mitochondrial protein is useful in detection of ongoing necrosis after RFA and provides pathological information on the validity of this procedure.

Original languageEnglish (US)
Pages (from-to)110-115
Number of pages6
JournalModern Pathology
Volume15
Issue number2
DOIs
StatePublished - 2002

Keywords

  • Hepatocellular carcinoma
  • Immunohistochemistry
  • Mitochondria
  • Ongoing necrosis
  • Radiofrequency ablation
  • ssDNA

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Fingerprint Dive into the research topics of 'Immunohistochemical detection of hepatocellular carcinoma in the setting of ongoing necrosis after radiofrequency ablation'. Together they form a unique fingerprint.

Cite this