Immunohistochemical detection of DNA alkylation adducts in rat and hamster liver after treatment with dimethylnitrosamine

M. Asamoto; A. M. Mikheev; Y. Z. Jiang; C. P. Wild; J. Hall; R. Montesano

doi:10.1016/S0232-1513(11)80004-6

Immunohistochemical detection of DNA alkylation adducts in rat and hamster liver after treatment with dimethylnitrosamine

M. Asamoto, A. M. Mikheev, Y. Z. Jiang, C. P. Wild, J. Hall, R. Montesano

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17 Scopus citations

Abstract

Monoclonal and polyclonal antibodies specific for methylation adducts have been applied in an immunohistochemical study of DNA damage in rat and hamster liver following exposure to dimethylnitrosamine. The approach was validated, for frozen and paraffin-embedded sections, by comparison with biochemical data on adduct levels in whole tissues or specific cell populations in the same experimental systems. The potential application of this method to human exposure assessment is discussed.

Original language	English (US)
Pages (from-to)	71-78
Number of pages	8
Journal	Experimental Pathology
Volume	41
Issue number	2
DOIs	https://doi.org/10.1016/S0232-1513(11)80004-6
State	Published - 1991

Keywords

DNA alkylation
dimethylnitrosamine
immunohistochemistry
immunohistochemistry, DNA
liver, dimethylnitrosamine

ASJC Scopus subject areas

Pathology and Forensic Medicine

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10.1016/S0232-1513(11)80004-6

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title = "Immunohistochemical detection of DNA alkylation adducts in rat and hamster liver after treatment with dimethylnitrosamine",

abstract = "Monoclonal and polyclonal antibodies specific for methylation adducts have been applied in an immunohistochemical study of DNA damage in rat and hamster liver following exposure to dimethylnitrosamine. The approach was validated, for frozen and paraffin-embedded sections, by comparison with biochemical data on adduct levels in whole tissues or specific cell populations in the same experimental systems. The potential application of this method to human exposure assessment is discussed.",

keywords = "DNA alkylation, dimethylnitrosamine, immunohistochemistry, immunohistochemistry, DNA, liver, dimethylnitrosamine",

author = "M. Asamoto and Mikheev, \{A. M.\} and Jiang, \{Y. Z.\} and Wild, \{C. P.\} and J. Hall and R. Montesano",

year = "1991",

doi = "10.1016/S0232-1513(11)80004-6",

language = "English (US)",

volume = "41",

pages = "71--78",

journal = "Experimental Pathology",

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T1 - Immunohistochemical detection of DNA alkylation adducts in rat and hamster liver after treatment with dimethylnitrosamine

AU - Asamoto, M.

AU - Mikheev, A. M.

AU - Jiang, Y. Z.

AU - Wild, C. P.

AU - Hall, J.

AU - Montesano, R.

PY - 1991

Y1 - 1991

N2 - Monoclonal and polyclonal antibodies specific for methylation adducts have been applied in an immunohistochemical study of DNA damage in rat and hamster liver following exposure to dimethylnitrosamine. The approach was validated, for frozen and paraffin-embedded sections, by comparison with biochemical data on adduct levels in whole tissues or specific cell populations in the same experimental systems. The potential application of this method to human exposure assessment is discussed.

AB - Monoclonal and polyclonal antibodies specific for methylation adducts have been applied in an immunohistochemical study of DNA damage in rat and hamster liver following exposure to dimethylnitrosamine. The approach was validated, for frozen and paraffin-embedded sections, by comparison with biochemical data on adduct levels in whole tissues or specific cell populations in the same experimental systems. The potential application of this method to human exposure assessment is discussed.

KW - DNA alkylation

KW - dimethylnitrosamine

KW - immunohistochemistry

KW - immunohistochemistry, DNA

KW - liver, dimethylnitrosamine

UR - https://www.scopus.com/pages/publications/0026098937

UR - https://www.scopus.com/inward/citedby.url?scp=0026098937&partnerID=8YFLogxK

U2 - 10.1016/S0232-1513(11)80004-6

DO - 10.1016/S0232-1513(11)80004-6

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AN - SCOPUS:0026098937

SN - 0232-1513

VL - 41

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JO - Experimental Pathology

JF - Experimental Pathology

IS - 2

ER -

Immunohistochemical detection of DNA alkylation adducts in rat and hamster liver after treatment with dimethylnitrosamine

Abstract

Keywords

ASJC Scopus subject areas

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