Immunohistochemical detection of DNA alkylation adducts in rat and hamster liver after treatment with dimethylnitrosamine

M. Asamoto; Andrei M Mikheev; Y. Z. Jiang; C. P. Wild; J. Hall; R. Montesano

doi:10.1016/S0232-1513(11)80004-6

Immunohistochemical detection of DNA alkylation adducts in rat and hamster liver after treatment with dimethylnitrosamine

M. Asamoto, Andrei M Mikheev, Y. Z. Jiang, C. P. Wild, J. Hall, R. Montesano

Research output: Contribution to journal › Article

17 Scopus citations

Abstract

Monoclonal and polyclonal antibodies specific for methylation adducts have been applied in an immunohistochemical study of DNA damage in rat and hamster liver following exposure to dimethylnitrosamine. The approach was validated, for frozen and paraffin-embedded sections, by comparison with biochemical data on adduct levels in whole tissues or specific cell populations in the same experimental systems. The potential application of this method to human exposure assessment is discussed.

Original language	English (US)
Pages (from-to)	71-78
Number of pages	8
Journal	Experimental Pathology
Volume	41
Issue number	2
DOIs	https://doi.org/10.1016/S0232-1513(11)80004-6
State	Published - Jan 1 1991

Keywords

DNA alkylation
dimethylnitrosamine
immunohistochemistry
immunohistochemistry, DNA
liver, dimethylnitrosamine

ASJC Scopus subject areas

Pathology and Forensic Medicine

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10.1016/S0232-1513(11)80004-6

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title = "Immunohistochemical detection of DNA alkylation adducts in rat and hamster liver after treatment with dimethylnitrosamine",

abstract = "Monoclonal and polyclonal antibodies specific for methylation adducts have been applied in an immunohistochemical study of DNA damage in rat and hamster liver following exposure to dimethylnitrosamine. The approach was validated, for frozen and paraffin-embedded sections, by comparison with biochemical data on adduct levels in whole tissues or specific cell populations in the same experimental systems. The potential application of this method to human exposure assessment is discussed.",

keywords = "DNA alkylation, dimethylnitrosamine, immunohistochemistry, immunohistochemistry, DNA, liver, dimethylnitrosamine",

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AU - Asamoto, M.

AU - Mikheev, Andrei M

AU - Jiang, Y. Z.

AU - Wild, C. P.

AU - Hall, J.

AU - Montesano, R.

PY - 1991/1/1

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N2 - Monoclonal and polyclonal antibodies specific for methylation adducts have been applied in an immunohistochemical study of DNA damage in rat and hamster liver following exposure to dimethylnitrosamine. The approach was validated, for frozen and paraffin-embedded sections, by comparison with biochemical data on adduct levels in whole tissues or specific cell populations in the same experimental systems. The potential application of this method to human exposure assessment is discussed.

AB - Monoclonal and polyclonal antibodies specific for methylation adducts have been applied in an immunohistochemical study of DNA damage in rat and hamster liver following exposure to dimethylnitrosamine. The approach was validated, for frozen and paraffin-embedded sections, by comparison with biochemical data on adduct levels in whole tissues or specific cell populations in the same experimental systems. The potential application of this method to human exposure assessment is discussed.

KW - DNA alkylation

KW - dimethylnitrosamine

KW - immunohistochemistry

KW - immunohistochemistry, DNA

KW - liver, dimethylnitrosamine

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