Monoclonal and polyclonal antibodies specific for methylation adducts have been applied in an immunohistochemical study of DNA damage in rat and hamster liver following exposure to dimethylnitrosamine. The approach was validated, for frozen and paraffin-embedded sections, by comparison with biochemical data on adduct levels in whole tissues or specific cell populations in the same experimental systems. The potential application of this method to human exposure assessment is discussed.
- DNA alkylation
- immunohistochemistry, DNA
- liver, dimethylnitrosamine
ASJC Scopus subject areas
- Pathology and Forensic Medicine