Immunohistochemical detection of DNA alkylation adducts in rat and hamster liver after treatment with dimethylnitrosamine

M. Asamoto, Andrei M Mikheev, Y. Z. Jiang, C. P. Wild, J. Hall, R. Montesano

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

Monoclonal and polyclonal antibodies specific for methylation adducts have been applied in an immunohistochemical study of DNA damage in rat and hamster liver following exposure to dimethylnitrosamine. The approach was validated, for frozen and paraffin-embedded sections, by comparison with biochemical data on adduct levels in whole tissues or specific cell populations in the same experimental systems. The potential application of this method to human exposure assessment is discussed.

Original languageEnglish (US)
Pages (from-to)71-78
Number of pages8
JournalExperimental Pathology
Volume41
Issue number2
DOIs
StatePublished - Jan 1 1991

Keywords

  • DNA alkylation
  • dimethylnitrosamine
  • immunohistochemistry
  • immunohistochemistry, DNA
  • liver, dimethylnitrosamine

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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