Immunohistochemical characterization of guided bone formation by a biodegradable tissue engineering scaffold in a healing tooth socket of a rabbit model

J. P. Fisher, Z. Lalani, N. Demian, M. E K Wong, A. G. Mikos

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Abstract

We have developed a novel, biocompatible, biodegradable tissue engineering scaffold that has been shown to facilitate bone formation in vivo. The process of bone formation within this scaffold, however, is unknown at a molecular level. To study this process, a rabbit's four healing incisor sockets (two mandibular and two maxillar) that remain after tooth extraction were used as a bone defect model. One socket was left empty, while the remaining three were filled with crosslinked polymer networks formed from either the hydrophobic polymer poly(propylene fumarate) (PPF), the hydrophilic oligomer oligo(poly(ethylene glycol) fumarate) (OPF), or PPF with adsorbed transforming growth factor - β1 (PPF+TGF-β1). At 4 days, 1 week, 2 weeks, 4 weeks, 8 weeks, and 16 weeks both the mandible and maxilla were removed and prepared for histological analysis. Using immunohistochemical techniques, frozen sections were stained for the presence of TGF-β1, platelet derived growth factor (PDGF), fibroblast growth factor - 2 (FGF-2), vascular endothelial growth factor (VEGF), and bone morphogenetic protein - 2 (BMP-2). Results indicate the effect of differing biomaterial properties upon bone formation as described by the spatial and temporal development of those growth factors thought to be intimately involved in the process.

Original languageEnglish (US)
Title of host publicationAnnual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
Pages827-828
Number of pages2
Volume1
StatePublished - 2002
EventProceedings of the 2002 IEEE Engineering in Medicine and Biology 24th Annual Conference and the 2002 Fall Meeting of the Biomedical Engineering Society (BMES / EMBS) - Houston, TX, United States
Duration: Oct 23 2002Oct 26 2002

Other

OtherProceedings of the 2002 IEEE Engineering in Medicine and Biology 24th Annual Conference and the 2002 Fall Meeting of the Biomedical Engineering Society (BMES / EMBS)
CountryUnited States
CityHouston, TX
Period10/23/0210/26/02

Keywords

  • Bone formation
  • Immunohistochemistry
  • Scaffold
  • Tissue engineering

ASJC Scopus subject areas

  • Bioengineering

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