TY - JOUR
T1 - Immunoglobulin E in chronic middle ear effusions
T2 - Comparison of rist, prist, and RIA techniques
AU - Lewis, Daniel M.
AU - Schram, James L.
AU - Birck, Herbert G.
AU - Lim, David J.
AU - Gleich, Gerald
N1 - Copyright:
Copyright 2016 Elsevier B.V., All rights reserved.
PY - 1978/3
Y1 - 1978/3
N2 - To investigate the possible role of allergy in otitis media with effusion (OME), the immunoglobulin E (IgE) content of 138 middle ear effusions (MEE) and paired serum samples from patients with chronic otitis media with effusion was determined. The initial 62 paired specimens were assayed for IgE by the radioimmunosorbent test (RIST), while the later 76 paired specimens were assayed for IgE by the paper radioimmunosorbent test (PRIST). When the results obtained by these two techniques were compared, it was noted that the PRIST procedure gave significantly lower IgE values for effusions than the RIST method. When the effusion-to-serum ratios (E/S ratios) were computed from the PRIST data, the E/S ratio was less than one, while RIST data gave an E/S ratio greater than one. The results obtained with the PRIST procedure were confirmed by double antibody radioimmunoassay for IgE. Thus, the PRIST procedure appears to measure the IgE content of MEE more accurately, and the results obtained by this procedure fail to support the concept of allergy as a major causative factor in OME.
AB - To investigate the possible role of allergy in otitis media with effusion (OME), the immunoglobulin E (IgE) content of 138 middle ear effusions (MEE) and paired serum samples from patients with chronic otitis media with effusion was determined. The initial 62 paired specimens were assayed for IgE by the radioimmunosorbent test (RIST), while the later 76 paired specimens were assayed for IgE by the paper radioimmunosorbent test (PRIST). When the results obtained by these two techniques were compared, it was noted that the PRIST procedure gave significantly lower IgE values for effusions than the RIST method. When the effusion-to-serum ratios (E/S ratios) were computed from the PRIST data, the E/S ratio was less than one, while RIST data gave an E/S ratio greater than one. The results obtained with the PRIST procedure were confirmed by double antibody radioimmunoassay for IgE. Thus, the PRIST procedure appears to measure the IgE content of MEE more accurately, and the results obtained by this procedure fail to support the concept of allergy as a major causative factor in OME.
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U2 - 10.1177/000348947808700209
DO - 10.1177/000348947808700209
M3 - Article
C2 - 646287
AN - SCOPUS:0018090701
SN - 0003-4894
VL - 87
SP - 197
EP - 201
JO - Annals of Otology, Rhinology & Laryngology
JF - Annals of Otology, Rhinology & Laryngology
IS - 2
ER -