Immunocytochemical study of the glucocorticoid receptor in rat liver nuclei after hyperthermic stress

Aleksandra Čvoro; Aleksandra Korać; Gordana Matić

doi:10.1016/S1065-6995(03)00019-2

Immunocytochemical study of the glucocorticoid receptor in rat liver nuclei after hyperthermic stress

Aleksandra Čvoro, Aleksandra Korać, Gordana Matić

Academic Institute

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

The presence of glucocorticoid receptors (GR) in rat liver nuclei over a 24 h time period following hyperthermic stress at 41°C was immunocytologically studied using unfixed nuclear smears. Liver nuclei in unstressed animals were found to be immunonegative for GR. However, intense GR immunopositivity followed by a subsequent gradual decrease in receptor levels was observed in the nuclei of test animals during the first 2 h after stress. This stress-related increase in the receptor nuclear level was greater than the increase seen after dexamethasone administration. These results suggest that hyperthermic stress could potentiate the hormonal stimulation of receptor nuclear translocation.

Original language	English (US)
Pages (from-to)	403-407
Number of pages	5
Journal	Cell Biology International
Volume	27
Issue number	5
DOIs	https://doi.org/10.1016/S1065-6995(03)00019-2
State	Published - May 2003

Keywords

Glucocorticoid receptor
Hyperthermic stress
Immunocytochemistry

ASJC Scopus subject areas

Cell Biology

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10.1016/S1065-6995(03)00019-2

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title = "Immunocytochemical study of the glucocorticoid receptor in rat liver nuclei after hyperthermic stress",

abstract = "The presence of glucocorticoid receptors (GR) in rat liver nuclei over a 24 h time period following hyperthermic stress at 41°C was immunocytologically studied using unfixed nuclear smears. Liver nuclei in unstressed animals were found to be immunonegative for GR. However, intense GR immunopositivity followed by a subsequent gradual decrease in receptor levels was observed in the nuclei of test animals during the first 2 h after stress. This stress-related increase in the receptor nuclear level was greater than the increase seen after dexamethasone administration. These results suggest that hyperthermic stress could potentiate the hormonal stimulation of receptor nuclear translocation.",

keywords = "Glucocorticoid receptor, Hyperthermic stress, Immunocytochemistry",

author = "Aleksandra {\v C}voro and Aleksandra Kora{\'c} and Gordana Mati{\'c}",

note = "Funding Information: This work was supported by the Ministry for Science, Technologies and Development of the Republic of Serbia, Yugoslavia, Grants #1654 and #1550. Copyright: Copyright 2008 Elsevier B.V., All rights reserved.",

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AU - Čvoro, Aleksandra

AU - Korać, Aleksandra

AU - Matić, Gordana

N1 - Funding Information: This work was supported by the Ministry for Science, Technologies and Development of the Republic of Serbia, Yugoslavia, Grants #1654 and #1550. Copyright: Copyright 2008 Elsevier B.V., All rights reserved.

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N2 - The presence of glucocorticoid receptors (GR) in rat liver nuclei over a 24 h time period following hyperthermic stress at 41°C was immunocytologically studied using unfixed nuclear smears. Liver nuclei in unstressed animals were found to be immunonegative for GR. However, intense GR immunopositivity followed by a subsequent gradual decrease in receptor levels was observed in the nuclei of test animals during the first 2 h after stress. This stress-related increase in the receptor nuclear level was greater than the increase seen after dexamethasone administration. These results suggest that hyperthermic stress could potentiate the hormonal stimulation of receptor nuclear translocation.

AB - The presence of glucocorticoid receptors (GR) in rat liver nuclei over a 24 h time period following hyperthermic stress at 41°C was immunocytologically studied using unfixed nuclear smears. Liver nuclei in unstressed animals were found to be immunonegative for GR. However, intense GR immunopositivity followed by a subsequent gradual decrease in receptor levels was observed in the nuclei of test animals during the first 2 h after stress. This stress-related increase in the receptor nuclear level was greater than the increase seen after dexamethasone administration. These results suggest that hyperthermic stress could potentiate the hormonal stimulation of receptor nuclear translocation.

KW - Glucocorticoid receptor

KW - Hyperthermic stress

KW - Immunocytochemistry

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JF - Cell Biology International

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Immunocytochemical study of the glucocorticoid receptor in rat liver nuclei after hyperthermic stress

Abstract

Keywords

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