Immunocytochemical localization of glucocorticoid receptor in human gingival fibroblasts and evidence for a colocalization of glucocorticoid receptor with cytoplasmic microtubules

G. Akner, K. G. Sundqvist, M. Denis, A. C. Wikstrom, Jan-Ake Gustafsson

Research output: Contribution to journalArticle

48 Scopus citations

Abstract

The cellular distribution of the glucocorticoid receptor (GR) in relation to various intracellular and plasma membrane structures in human fibroblasts was studied using indirect immunofluorescence techniques with monoclonal and polyclonal antibodies. During interphase, GR was located predominantly in the cytoplasm, showing a similar pattern as tubulin. In mitotic cells, GR and tubulin were localized in mitotic spindles and in telophase midbodies. Colchicine and vinblastine induced a similar redistribution of GR and tubulin to the cell periphery. This redistribution was reversible for colchicine but not for vinblastine. Vinblastine also induced paracrystals containing GR and tubulin. These results support the hypothesis that GR interacts in vivo with cytoplasmic microtubules.

Original languageEnglish (US)
Pages (from-to)390-401
Number of pages12
JournalEuropean Journal of Cell Biology
Volume53
Issue number2
StatePublished - Dec 1 1990

Keywords

  • Colocalization
  • Fibroblasts
  • Glucocorticoid receptor
  • Tubulin

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Fingerprint Dive into the research topics of 'Immunocytochemical localization of glucocorticoid receptor in human gingival fibroblasts and evidence for a colocalization of glucocorticoid receptor with cytoplasmic microtubules'. Together they form a unique fingerprint.

Cite this