Immunocytochemical detection of Salmonella Gallinarum and Salmonella Pullorum in experimentally infected chickens

Prasanna Krishnamurthy, O. P. Paliwal, Ram Kumar, B. N. Trifathi

Research output: Contribution to journalArticle

Abstract

Experimental fowl typhoid (FT) and pullorum disease (PD) were induced by intracrop inoculation or S. Gallinarum and S. Pullorum, respectively, in 1-week-old White Leghorn chicks. The organisms were reisolated by cloacal swab culturing, and their localization in the tissues was detected by immunoperoxidase (IPT) and immunofluorescence (IF) techniques at various intervals. The antigens were also detected in tissues, viz. crop, liver, duodenum, ileocaecal junction and caecum by immunocytochemistry during the intervals when the organisms ceased to be excreted in the faeces and thus can be used to detect the carrier status of the chickens.

Original languageEnglish (US)
Pages (from-to)113-116
Number of pages4
JournalIndian Journal of Animal Sciences
Volume72
Issue number2
StatePublished - Feb 1 2002

Keywords

  • Chickens
  • Fowl typhoid
  • Immunocytology
  • Immunofluorescence
  • Immunoperoxidase
  • Pullorum disease
  • Salmonella Gallinarum
  • Salmonella Pullorum
  • Salmonellosis

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)

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