Immortalization of normal adult human middle ear epithelial cells using a retrovirus containing the E6/E7 genes of human papillomavirus type 16

Young Myoung Chun, Sung Kyun Moon, Derald E. Brackmann, Haa Yung Lee, Johng S. Rhim, Paul Webster, David J. Lim

Research output: Contribution to journalArticlepeer-review

65 Scopus citations

Abstract

A human middle ear epithelial cell line (HMEEC-1) was established using human papillomavirus E6/E7 genes. HMEEC-1 has remained morphologically and phenotypically stable, even after 50 passages. The cells are anchorage-dependent and nontumorigenic when injected into nude mice. This cell line thus provides a new tool for the study of normal cell biology and the pathological processes associated with the epithelial cells of the middle ear in otitis media. HMEEC-1 will also be useful in the search for new drugs and biological agents for the treatment of otitis media.

Original languageEnglish (US)
Pages (from-to)507-517
Number of pages11
JournalAnnals of Otology, Rhinology and Laryngology
Volume111
Issue number6
DOIs
StatePublished - 2002

Keywords

  • Human middle ear epithelial cell
  • Human papillomavirus type 16
  • Immortalization

ASJC Scopus subject areas

  • Otorhinolaryngology

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