Immortalization of normal adult human middle ear epithelial cells using a retrovirus containing the E6/E7 genes of human papillomavirus type 16

Young Myoung Chun; Sung Kyun Moon; Derald E. Brackmann; Haa Yung Lee; Johng S. Rhim; Paul Webster; David J. Lim

doi:10.1177/000348940211100606

Immortalization of normal adult human middle ear epithelial cells using a retrovirus containing the E6/E7 genes of human papillomavirus type 16

Young Myoung Chun, Sung Kyun Moon, Derald E. Brackmann, Haa Yung Lee, Johng S. Rhim, Paul Webster, David J. Lim

Research output: Contribution to journal › Article › peer-review

75 Scopus citations

Abstract

A human middle ear epithelial cell line (HMEEC-1) was established using human papillomavirus E6/E7 genes. HMEEC-1 has remained morphologically and phenotypically stable, even after 50 passages. The cells are anchorage-dependent and nontumorigenic when injected into nude mice. This cell line thus provides a new tool for the study of normal cell biology and the pathological processes associated with the epithelial cells of the middle ear in otitis media. HMEEC-1 will also be useful in the search for new drugs and biological agents for the treatment of otitis media.

Original language	English (US)
Pages (from-to)	507-517
Number of pages	11
Journal	Annals of Otology, Rhinology and Laryngology
Volume	111
Issue number	6
DOIs	https://doi.org/10.1177/000348940211100606
State	Published - 2002

Keywords

Human middle ear epithelial cell
Human papillomavirus type 16
Immortalization

ASJC Scopus subject areas

Otorhinolaryngology

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10.1177/000348940211100606

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title = "Immortalization of normal adult human middle ear epithelial cells using a retrovirus containing the E6/E7 genes of human papillomavirus type 16",

abstract = "A human middle ear epithelial cell line (HMEEC-1) was established using human papillomavirus E6/E7 genes. HMEEC-1 has remained morphologically and phenotypically stable, even after 50 passages. The cells are anchorage-dependent and nontumorigenic when injected into nude mice. This cell line thus provides a new tool for the study of normal cell biology and the pathological processes associated with the epithelial cells of the middle ear in otitis media. HMEEC-1 will also be useful in the search for new drugs and biological agents for the treatment of otitis media.",

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AU - Chun, Young Myoung

AU - Moon, Sung Kyun

AU - Brackmann, Derald E.

AU - Lee, Haa Yung

AU - Rhim, Johng S.

AU - Webster, Paul

AU - Lim, David J.

PY - 2002

Y1 - 2002

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AB - A human middle ear epithelial cell line (HMEEC-1) was established using human papillomavirus E6/E7 genes. HMEEC-1 has remained morphologically and phenotypically stable, even after 50 passages. The cells are anchorage-dependent and nontumorigenic when injected into nude mice. This cell line thus provides a new tool for the study of normal cell biology and the pathological processes associated with the epithelial cells of the middle ear in otitis media. HMEEC-1 will also be useful in the search for new drugs and biological agents for the treatment of otitis media.

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KW - Human papillomavirus type 16

KW - Immortalization

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Immortalization of normal adult human middle ear epithelial cells using a retrovirus containing the E6/E7 genes of human papillomavirus type 16

Abstract

Keywords

ASJC Scopus subject areas

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