Immobilized cytokines as biomaterials for manufacturing immune cell based vaccines

Claude Leclerc, Claudia Brose, Clémence Nouzé, Fransisca Leonard, Laleh Majlessi, Sybille Becker, Hagen Von Briesen, Richard Lo-Man

Research output: Contribution to journalArticlepeer-review

11 Scopus citations


Manufacturing of bioactive cell culture substrates represents a major challenge for the development of cell therapy for tissue repair and immune treatment of cancers, infectious diseases, or immunodeficiencies. In this context, we evaluated the capacity of several differentiation factors, including Granulocyte Macrophage Colony Stimulating Factor (GM-CSF) and Macrophage Colony Stimulating Factor (M-CSF), to drive differentiation of primary cell cultures, once immobilized on surfaces. We show that covalently immobilized signal factors fully retain their biological properties and efficiently promote differentiation of mouse and/or human precursor cells leading to the production of dendritic cells and macrophages. For GM-CSF, we also show that the efficiency of receptor signaling is comparable using either soluble or tethered molecules. Such artificial bioactive interfaces are suitable for the development and automated production of cell-based vaccines and therapies.

Original languageEnglish (US)
Pages (from-to)1033-1040
Number of pages8
JournalJournal of Biomedical Materials Research - Part A
Issue number4
StatePublished - Sep 15 2008


  • Bioactive material
  • Cytokine
  • Immobilization
  • Immune cell differentiation

ASJC Scopus subject areas

  • Ceramics and Composites
  • Biomaterials
  • Biomedical Engineering
  • Metals and Alloys


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