TY - JOUR
T1 - Imaging of atherosclerosis in apoliprotein e knockout mice
T2 - targeting of a folate-conjugated radiopharmaceutical to activated macrophages.
AU - Ayala-López, Wilfredo
AU - Xia, Wei
AU - Varghese, Bindu
AU - Low, Philip S.
N1 - Copyright:
This record is sourced from MEDLINE/PubMed, a database of the U.S. National Library of Medicine
PY - 2010/5
Y1 - 2010/5
N2 - Early detection of heart disease is essential for the implementation of intervention strategies that reduce the risk of cardiovascular events. Radioimaging methods that have been explored for this purpose include (18)F-FDG, which measures sites of elevated metabolic activity; (99m)Tc-annexin A5, which reveals regions of enhanced apoptosis and thrombosis; and (99m)Tc-labeled anti-lectinlike oxidized low-density lipoprotein receptor 1 antibody, which detects the lectinlike oxidized low-density lipoprotein receptor 1 that is overexpressed on a variety of vasculature-associated cells. In this study, we examine the use of a folate-targeted chelate of (99m)Tc, termed (99m)Tc-EC20, for imaging of folate receptor (FR)-expressing macrophages that accumulate in atherosclerotic plaques, internalize cholesterol-rich lipoprotein particles, and evolve into foam cells that form components of vulnerable atherosclerotic lesions. METHODS: (99m)Tc-EC20 was injected into apoliprotein E knockout (apoE-/-) mice fed a normal or Western (high-fat) diet for 25 wk and imaged by gamma-scintigraphy. Treated mice were also dissected, and radioactivities in excised aortas were quantified by gamma-counting and imaged by autoradiography. The role of FR-expressing macrophages in uptake of (99m)Tc-EC20 was also examined by comparing images of apoE-/- mice before and after treatment with clodronate liposomes to deplete tissue macrophages, comparing the sites of (99m)Tc-EC20 enrichment with sites of macrophage accumulation in thin sections of atherosclerotic tissues, and examining the expression of FRs on atherosclerotic plaque-derived macrophages by flow cytometry. RESULTS: ApoE-/- mice on Western chow exhibited significantly greater accumulation of (99m)Tc-EC20 in atherosclerotic lesions than their counterparts on normal chow. The aortas of apoE-/- mice on a Western diet demonstrated greater numbers of FR-positive macrophages by flow cytometry than did those of apoE-/- mice on a normal diet. Clodronate liposome treatment significantly reduced the accumulation of (99m)Tc-EC20 in atherosclerotic tissues, suggesting that macrophages or monocytes are responsible for uptake of the folate-linked radioimaging agent. Histologic and autoradiographic analysis of tissue sections demonstrated that macrophage accumulation correlated with regions of (99m)Tc-EC20 uptake. CONCLUSION: (99m)Tc-EC20 can be used for the imaging of atherosclerosis by selectively targeting FR-positive activated macrophages.
AB - Early detection of heart disease is essential for the implementation of intervention strategies that reduce the risk of cardiovascular events. Radioimaging methods that have been explored for this purpose include (18)F-FDG, which measures sites of elevated metabolic activity; (99m)Tc-annexin A5, which reveals regions of enhanced apoptosis and thrombosis; and (99m)Tc-labeled anti-lectinlike oxidized low-density lipoprotein receptor 1 antibody, which detects the lectinlike oxidized low-density lipoprotein receptor 1 that is overexpressed on a variety of vasculature-associated cells. In this study, we examine the use of a folate-targeted chelate of (99m)Tc, termed (99m)Tc-EC20, for imaging of folate receptor (FR)-expressing macrophages that accumulate in atherosclerotic plaques, internalize cholesterol-rich lipoprotein particles, and evolve into foam cells that form components of vulnerable atherosclerotic lesions. METHODS: (99m)Tc-EC20 was injected into apoliprotein E knockout (apoE-/-) mice fed a normal or Western (high-fat) diet for 25 wk and imaged by gamma-scintigraphy. Treated mice were also dissected, and radioactivities in excised aortas were quantified by gamma-counting and imaged by autoradiography. The role of FR-expressing macrophages in uptake of (99m)Tc-EC20 was also examined by comparing images of apoE-/- mice before and after treatment with clodronate liposomes to deplete tissue macrophages, comparing the sites of (99m)Tc-EC20 enrichment with sites of macrophage accumulation in thin sections of atherosclerotic tissues, and examining the expression of FRs on atherosclerotic plaque-derived macrophages by flow cytometry. RESULTS: ApoE-/- mice on Western chow exhibited significantly greater accumulation of (99m)Tc-EC20 in atherosclerotic lesions than their counterparts on normal chow. The aortas of apoE-/- mice on a Western diet demonstrated greater numbers of FR-positive macrophages by flow cytometry than did those of apoE-/- mice on a normal diet. Clodronate liposome treatment significantly reduced the accumulation of (99m)Tc-EC20 in atherosclerotic tissues, suggesting that macrophages or monocytes are responsible for uptake of the folate-linked radioimaging agent. Histologic and autoradiographic analysis of tissue sections demonstrated that macrophage accumulation correlated with regions of (99m)Tc-EC20 uptake. CONCLUSION: (99m)Tc-EC20 can be used for the imaging of atherosclerosis by selectively targeting FR-positive activated macrophages.
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U2 - 10.2967/jnumed.109.071324
DO - 10.2967/jnumed.109.071324
M3 - Article
C2 - 20395331
AN - SCOPUS:77952295715
VL - 51
SP - 768
EP - 774
JO - Journal of Nuclear Medicine
JF - Journal of Nuclear Medicine
SN - 0161-5505
IS - 5
ER -