IL-10 improves lung injury and survival in Pseudomonas aeruginosa pneumonia

Teiji Sawa, David B. Corry, Michael A. Gropper, Maria Ohara, Kiyoyasu Kurahashi, Jeanine P. Wiener-Kronish

Research output: Contribution to journalArticlepeer-review

137 Scopus citations


Pseudomonas aeruginosa is the most frequent Gram-negative pathogen causing nosocomial pneumonia. Four different strains of P. aeruginosa (including three isogenic transposon mutants) were utilized in experiments in mice to characterize the specific patterns of cytokine generation in response to bacterial products and cytotoxicity. Intratracheal instillation of any of the strains led to the up-regulation of IL-1β, IL-6, and TNF-α mRNA. Instillation of the cytotoxic strains (PA103, PA103tox::Ω) led to IL-10 mRNA up-regulation in the lungs and increased concentrations of IL-10 in the blood. In contrast, the instillation of the noncytotoxic strains (PA01, PA103exsA::Ω) did not lead to an increase in IL-10 mRNA in the lungs or to an increase of IL-10 concentration in blood. IL-10 production appears to be a response to either cellular injury or to specific cytotoxic exoproducts produced by the bacteria. The systemic administration of rlL-10 significantly decreased the lung injury and the mortality in mice who had received the cytotoxic strains. The improvement in survival induced by administration of rlL-10 required the concomitant presence of IFN-γ, as blockade of IFN-γ with a neutralizing Ab led to 100% mortality, despite the administration of rlL-10. These results suggest that IL-10 is produced in response to specific bacterial products and that there is a potential role for IL-10 in the treatment of cytotoxic P. aeruginosa pneumonia.

Original languageEnglish (US)
Pages (from-to)2858-2866
Number of pages9
JournalJournal of Immunology
Issue number6
StatePublished - 1997

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology


Dive into the research topics of 'IL-10 improves lung injury and survival in Pseudomonas aeruginosa pneumonia'. Together they form a unique fingerprint.

Cite this