TY - JOUR
T1 - IgG1 + ovalbumin-specific B-cell transnuclear mice show class switch recombination in rare allelically included B cells
AU - Dougan, Stephanie K.
AU - Ogata, Souichi
AU - Hu, Chih Chi Andrew
AU - Grotenbreg, Gijsbert M.
AU - Guillen, Eduardo
AU - Jaenisch, Rudolf
AU - Ploegh, Hidde L.
PY - 2012/8/21
Y1 - 2012/8/21
N2 - We used somatic cell nuclear transfer (SCNT) to generate a mouse from the nucleus of an IgG1 + ovalbumin-specific B cell. The resulting OBI mice show generally normal B-cell development, with elevated percentages of marginal zone B cells and a reduction in B-1 B cells. Whereas OBI RAG1 -/- mice have exclusively IgG1 anti-ovalbumin in their serum, OBI mice show elevated levels of anti-ovalbumin of nearly all isotypes 3′ of the γ1 constant region in the IgH locus, indicating that class switch recombination (CSR) occurs in the absence of immunization with ovalbumin. This CSR is associated with the presence of IgM +IgG1 + double producer B cells that represent <1% of total B cells, accumulate in the peritoneal cavity, and account for near-normal levels of serum IgM and IgG3.
AB - We used somatic cell nuclear transfer (SCNT) to generate a mouse from the nucleus of an IgG1 + ovalbumin-specific B cell. The resulting OBI mice show generally normal B-cell development, with elevated percentages of marginal zone B cells and a reduction in B-1 B cells. Whereas OBI RAG1 -/- mice have exclusively IgG1 anti-ovalbumin in their serum, OBI mice show elevated levels of anti-ovalbumin of nearly all isotypes 3′ of the γ1 constant region in the IgH locus, indicating that class switch recombination (CSR) occurs in the absence of immunization with ovalbumin. This CSR is associated with the presence of IgM +IgG1 + double producer B cells that represent <1% of total B cells, accumulate in the peritoneal cavity, and account for near-normal levels of serum IgM and IgG3.
KW - Allelic exclusion
KW - Natural antibodies
KW - TN mice
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U2 - 10.1073/pnas.1210273109
DO - 10.1073/pnas.1210273109
M3 - Article
C2 - 22869725
AN - SCOPUS:84865279635
VL - 109
SP - 13739
EP - 13744
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
SN - 0027-8424
IS - 34
ER -