IgG1 + ovalbumin-specific B-cell transnuclear mice show class switch recombination in rare allelically included B cells

Stephanie K. Dougan, Souichi Ogata, Chih Chi Andrew Hu, Gijsbert M. Grotenbreg, Eduardo Guillen, Rudolf Jaenisch, Hidde L. Ploegh

Research output: Contribution to journalArticle

27 Scopus citations

Abstract

We used somatic cell nuclear transfer (SCNT) to generate a mouse from the nucleus of an IgG1 + ovalbumin-specific B cell. The resulting OBI mice show generally normal B-cell development, with elevated percentages of marginal zone B cells and a reduction in B-1 B cells. Whereas OBI RAG1 -/- mice have exclusively IgG1 anti-ovalbumin in their serum, OBI mice show elevated levels of anti-ovalbumin of nearly all isotypes 3′ of the γ1 constant region in the IgH locus, indicating that class switch recombination (CSR) occurs in the absence of immunization with ovalbumin. This CSR is associated with the presence of IgM +IgG1 + double producer B cells that represent <1% of total B cells, accumulate in the peritoneal cavity, and account for near-normal levels of serum IgM and IgG3.

Original languageEnglish (US)
Pages (from-to)13739-13744
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume109
Issue number34
DOIs
StatePublished - Aug 21 2012

Keywords

  • Allelic exclusion
  • Natural antibodies
  • TN mice

ASJC Scopus subject areas

  • General

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