In order to investigate the mechanisms of pregnancy associated with diabetes harm to gravida and fetal, The Kunming mice models for pregnancy associated with diabetes were simulated, The effects of glucose in different concentrations was detected on the growth of embryo cells in vitro culture. The effects of IGF-1 in different concentrations was determined on the development of blastocyst in hyperglycemic conditions (30.0 mol/L glucose) in vitro, as well as the apoptosis of embyro cells by using nuclear DNA double-dyed assays. Moreover, the expression of igf-1 and other genes associated with apoptosis in vitro embryo was detcted by Real-time quantitative PCR. The results showed that the total cell number of blastocyst fell off along with the increasing of glucose concentration. High concentrations of glucose (≥ 30 mmol/L) could significantly inhibit the growth of embryo cells (P < 0.01). The results of real-time quantitative PCR showed that the expression of igf-1 was down-regulated in mouse blastocyst with pregnancy associated with diabetes, and positively correlated with the concentration of glucose. The expressions of apoptosis-related genes bcl-2 and bcl-xl were up-regulated along with the increasing of IGF-1 concentration, while the p53 gene and the apoptosis-related gene Box were down-regulated. The embryo cells apoptosis had been gradually reduced with the increasing of IGF-I concentration in vitro, and in the IGF-1 concentration of 100 (xg/L, there was almost no apoptosis in embryo cells. These experiments demonstrated that hyperglycemic conditions in vitro can inhibit the growth and development of the embryo cells resulting in the down-regulated expression of igf-1, and IGF-1 could inhibit the apoptosis of blastocyst and be benefit for the growth of blastocyst.
- In vitro culture (ivc)
- Mouse embryos
- Pregnancy associated with diabetes
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