iDISCO Tissue Clearing Whole-Brain and Light Sheet Microscopy for High-Throughput Imaging in a Mouse Model of Traumatic Brain Injury

Hannah Flinn, Leonardo Cruz-Pineda, Laura Montier, Philip J. Horner, Sonia Villapol

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Immunolabeling-enabled imaging of solvent-cleared organs (iDISCO) (Renier N, Wu Z, Simon DJ, Yang J, Ariel P, Tessier-Lavigne M, Cell 159:896–910, 2014) aims to match the refractive index (RI) of tissue to the surrounding medium, thereby facilitating three-dimensional (3D) imaging and quantification of cellular points and tissue structures. Once cleared, transparent tissue samples allow for rapid imaging with no mechanical sectioning. This imaging technology enables us to visualize brain tissue in situ and quantify the morphology and extent of glial cell branches or neuronal processes extending from the epicenter of a traumatic brain injury (TBI). In this way, we can more accurately assess and quantify the damaging consequences of TBI not only in the impact region but also in the extended pericontusional regions.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press
Pages589-597
Number of pages9
Volume2761
DOIs
StatePublished - 2024

Publication series

NameMethods in molecular biology (Clifton, N.J.)
ISSN (Print)1064-3745

Keywords

  • 3D imaging
  • Immunolabeling-enabled imaging of solvent-cleared organs (iDISCO)
  • Light sheet microscopy
  • Tissue clearing
  • Whole-brain clearing
  • Solvents
  • Brain
  • Animals
  • Microscopy
  • Imaging, Three-Dimensional/methods
  • Mice
  • Brain Injuries, Traumatic/diagnostic imaging

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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