TY - JOUR
T1 - Idiotype immunization combined with granulocyte-macrophage colony- stimulating factor in myeloma patients induced type I, major histocompatibility complex-restricted, CD8- and CD4-specific T-cell responses
AU - Österborg, Anders
AU - Yi, Qing
AU - Henriksson, Lotta
AU - Fagerberg, Jan
AU - Bergenbrant, Susanne
AU - Jeddi-Tehrani, Mahmood
AU - Rudén, Ulla
AU - Lefvert, Ann Kari
AU - Holm, Göran
AU - Mellstedt, Håkan
N1 - Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 1998/4/1
Y1 - 1998/4/1
N2 - Idiotypic structures expressed on the myeloma Ig protein might be regarded as a tumor-specific antigen. Five patients with IgG myeloma were immunized with the purified serum M-component by repeated intradermal injections together with soluble granulocyte-macrophage colony-stimulating factor(GM-CSF). All patients developed an idiotype (Id)-specific T-cell Immunity, defined as blood T cells predominantly secreting interferon-γ (IFN-γ) and interleukin-2 (IL-2) (type I cells). Id-specific DNA synthesis was induced in one patient. Delayed-type hypersensitivity against the Id was not evoked. The specific IFN-γ/IL-2 T-cell response was inhibited (46% to 100%) by a major histocompatibility complex (MHC) class I monoclonal antibody (MoAb) in all five patients. A 5% to 37% inhibition by an MHC class II MoAb was seen in four patients. CD4+ as well as CD8+ T cells enriched by magnetic microbeads contained Id-specific cells. The T cells recognized peptides corresponding to the complementarity-determining regions 1, 2, and 3 of the heavy chain of the Id. There was a transient rise of B cells producing IgM anti-idiotypic antibodies in all patients. The results indicate that immunization of myeloma patients using the autologous M-component and soluble GM-CSF may evoke an Id-specific predominantly MHC class I-restricted type I T-cell response.
AB - Idiotypic structures expressed on the myeloma Ig protein might be regarded as a tumor-specific antigen. Five patients with IgG myeloma were immunized with the purified serum M-component by repeated intradermal injections together with soluble granulocyte-macrophage colony-stimulating factor(GM-CSF). All patients developed an idiotype (Id)-specific T-cell Immunity, defined as blood T cells predominantly secreting interferon-γ (IFN-γ) and interleukin-2 (IL-2) (type I cells). Id-specific DNA synthesis was induced in one patient. Delayed-type hypersensitivity against the Id was not evoked. The specific IFN-γ/IL-2 T-cell response was inhibited (46% to 100%) by a major histocompatibility complex (MHC) class I monoclonal antibody (MoAb) in all five patients. A 5% to 37% inhibition by an MHC class II MoAb was seen in four patients. CD4+ as well as CD8+ T cells enriched by magnetic microbeads contained Id-specific cells. The T cells recognized peptides corresponding to the complementarity-determining regions 1, 2, and 3 of the heavy chain of the Id. There was a transient rise of B cells producing IgM anti-idiotypic antibodies in all patients. The results indicate that immunization of myeloma patients using the autologous M-component and soluble GM-CSF may evoke an Id-specific predominantly MHC class I-restricted type I T-cell response.
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U2 - 10.1182/blood.v91.7.2459.2459_2459_2466
DO - 10.1182/blood.v91.7.2459.2459_2459_2466
M3 - Article
C2 - 9516146
AN - SCOPUS:0032055911
VL - 91
SP - 2459
EP - 2466
JO - Blood
JF - Blood
SN - 0006-4971
IS - 7
ER -