Identification of the cDNA for xlcaax-1, a membrane associated Xenopus maternal protein

Bramham A. Reddy, Malgorzata Kloc, Laurence D. Etkin

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

xlcaax-1 is a cDNA coding for a CAAX box containing protein in Xenopus that undergoes isoprenylation and palmitoylation. Here we report on the confirmation that this clone (formerly xlgv 7) codes for a 110 kDa membrane associated protein and not an 80 kDa nuclear protein as originally believed (1). The reason for the misidentification was the presence of a common epitope on these two proteins recognized by the monoclonal antibody 37-1A9. We clarified the discrepancy by raising polyclonal antibodies against the xlcaax-1 protein produced in a bacterial expression system and demonstrating that these antibodies only recognize the 110 kDa protein on western blots of oocyte extracts. During early development xlcaax-1 protein starts reaccumulating from the neurula stage. In the adult frog both the xlcaax-1 protein and its cognate mRNA are highly enriched in the kidney. Consistent with the presence of CAAX box at the C-terminus this protein is associated with the membranes in Xenopus tissue culture cells (XTC).

Original languageEnglish (US)
Pages (from-to)1635-1641
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume179
Issue number3
DOIs
StatePublished - Sep 30 1991

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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