Identification of proteins highly expressed in uterine fluid from mice with hydrometra

Per Antonson, Ivan Nalvarte, Mukesh Varshney, Li Xu, Sara H. Windahl, Patricia Humire, Claes Ohlsson, Jan Åke Gustafsson, Karin Dahlman-Wright

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Estrogen receptor alpha (ERα) is an important regulator of the estrous cycle and mice with global ERα deletion, as well as some conditional knockout mouse lines, have an interruption in the estrous cycle. In this study we observed that conditional ERα knockout mice where the Cre gene is regulated by the rat insulin promoter (RIP), RIP-Cre/ERαKO mice, have a 3.7-fold increase in serum 17β-estradiol levels, blocked estrous cycle, and develop a fluid-filled uterus (hydrometra). Using a proteomics approach, we identified three proteins, lactoferrin, complement C3 and chitinase 3-like protein 1 (CHI3L1), as highly expressed proteins in hydrometra fluid. The mRNA levels of the corresponding genes were more than 50-fold higher in RIP-Cre/ERαKO uterus compared to controls. High expression of CHI3L1 in the uterine fluid was not reflected as elevated levels in the serum. The high expression of lactoferrin, complement C3 and CHI3L1 in the uterine fluid, in association with elevated estrogen levels, prompted us to address if the expression of these genes is related to reproduction. However, gonadotropin treatment of mice reduced the uterine expression of these genes in a model of in vitro fertilization. Our findings identify lactoferrin, complement C3 and CHI3L1 as highly expressed proteins in hydrometra fluid in association with chronically elevated serum estradiol levels.

Original languageEnglish (US)
Pages (from-to)650-655
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume466
Issue number4
DOIs
StatePublished - Oct 30 2015

Keywords

  • Estrogen
  • Estrogen receptor
  • Knockout
  • Proteomics
  • Uterus

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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