Identification of proteins associated with proliferation inhibition effects by DADS in SW480 cell line

Aim: To investigate the arrest effect of diallyl disulfide (DADS) in the cell cycle of human nasopharyngeal carcinoma SW480 cell line and its molecular mechanism. Mothdes: The growth inhibition effect of DADS of different concentrations on SW480 cell line was measured by MTT assay and cell counting. Phase distribution of cell cycle was analyzed by flow cytometry. Expression of Ubiquitin and FKBP was determined by Western blot. Results: The MTT assay showed that DADS inhibited growth of SW480 cells significantly in a dose-dependent manner. Adding 25, 35, 50 and 70 mg·L^-1 DADS for 48 hours, SW480 cell growth was suppressed by 18.67%, 33.02%, 49.12% and 66.86%, respectively. There were significant differences between the treated and controlled cases (P < 0.05). Flow cytometry analysis revealed that SW480 cells treated with 50 mg·L^-1 DADS increased the percentage of cells in the G₂/M phase. Ubiquitin was increased; FKBP was decreased significantly in SW480 treated with 50 mg·L^-1 DADS using Western blot. The differences were more than two times. Conclusion: The antiproliferation property of diallyl disulfide (DADS) in cultured human colon carcinoma SW480 cell line is related to its ability to arrest G₂/M phrase through over-regulation of Ubiquitin and down-regulation of FKBP.