Identification of point mutations in clinical staphylococcus aureus strains that produce small-colony variants auxotrophic for menadione

Melissa A. Dean, Randall J. Olsen, S. Wesley Long, Adriana E. Rosato, James M. Musser

Research output: Contribution to journalArticle

31 Scopus citations

Abstract

Staphylococcus aureus small-colony variants (SCVs) are implicated in chronic and relapsing infections that are difficult to diagnose and treat. Despite many years of study, the underlying molecular mechanisms and virulence effect of the small-colony phenotype remain incompletely understood. We sequenced the genomes of five S. aureus SCV strains recovered from human patients and discovered previously unidentified nonsynonymous point mutations in three genes encoding proteins in the menadione biosynthesis pathway. Analysis of genetic revertants and complementation with wild-type alleles confirmed that these mutations caused the SCV phenotype and decreased virulence for mice.

Original languageEnglish (US)
Pages (from-to)1600-1605
Number of pages6
JournalInfection and Immunity
Volume82
Issue number4
DOIs
StatePublished - Apr 2014

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

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