Identification of new genes differentially expressed in coronary artery disease by expression profiling

Stephen R. Archacki, George Angheloiu, Xiao Li Tian, Fen Lai Tan, Nick DiPaola, Gong Qing Shen, Christine Moravec, Stephen Ellis, Eric J. Topol, Qing Wang

Research output: Contribution to journalArticle

64 Scopus citations

Abstract

Genetics factors increase the risk to coronary artery disease (CAD). To date, a limited number of genes that potentially contribute to development of CAD have been identified. In this study, we have performed large-scale gene expression analysis of ∼12,000 human genes in nine severely atherosclerotic and six non-atherosclerotic human coronary arteries using oligonucleotide microarrays. Fifty-six genes showed differential expression in atherosclerotic coronary artery tissues; expression of 55 genes was increased in atherosclerotic coronary arteries, whereas only one gene, GST, encoding a reducing agent, showed downregulated expression. The expression data of selected genes were validated by quantitative RT-PCR analysis as well as immunostaining. The associations of 49 genes with CAD appear to be novel, and they include genes encoding ICAM-2, PIM-2, ECGF1, fusin, B cell activator (BL34, GOS8), Rho GTPase activating protein-4, retinoic acid receptor responder, β2-arrestin, membrane aminopeptidase, cathepsins K and H, MIR-7, TNF-α-induced protein 2 (B94), and flavocytochrome 558. In conclusion, we have identified 56 genes whose expression is associated with CAD, and 49 of them may represent new genes linked to CAD.

Original languageEnglish (US)
Pages (from-to)65-74
Number of pages10
JournalPhysiological Genomics
Volume15
DOIs
StatePublished - Jan 2004

Keywords

  • Array
  • Atherosclerosis
  • Coronary artery disease/myocardial infarction
  • Gene expression
  • Sudden death

ASJC Scopus subject areas

  • Physiology
  • Genetics

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