TY - JOUR
T1 - Identification of new genes differentially expressed in coronary artery disease by expression profiling
AU - Archacki, Stephen R.
AU - Angheloiu, George
AU - Tian, Xiao Li
AU - Tan, Fen Lai
AU - DiPaola, Nick
AU - Shen, Gong Qing
AU - Moravec, Christine
AU - Ellis, Stephen
AU - Topol, Eric J.
AU - Wang, Qing
N1 - Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2004/1
Y1 - 2004/1
N2 - Genetics factors increase the risk to coronary artery disease (CAD). To date, a limited number of genes that potentially contribute to development of CAD have been identified. In this study, we have performed large-scale gene expression analysis of ∼12,000 human genes in nine severely atherosclerotic and six non-atherosclerotic human coronary arteries using oligonucleotide microarrays. Fifty-six genes showed differential expression in atherosclerotic coronary artery tissues; expression of 55 genes was increased in atherosclerotic coronary arteries, whereas only one gene, GST, encoding a reducing agent, showed downregulated expression. The expression data of selected genes were validated by quantitative RT-PCR analysis as well as immunostaining. The associations of 49 genes with CAD appear to be novel, and they include genes encoding ICAM-2, PIM-2, ECGF1, fusin, B cell activator (BL34, GOS8), Rho GTPase activating protein-4, retinoic acid receptor responder, β2-arrestin, membrane aminopeptidase, cathepsins K and H, MIR-7, TNF-α-induced protein 2 (B94), and flavocytochrome 558. In conclusion, we have identified 56 genes whose expression is associated with CAD, and 49 of them may represent new genes linked to CAD.
AB - Genetics factors increase the risk to coronary artery disease (CAD). To date, a limited number of genes that potentially contribute to development of CAD have been identified. In this study, we have performed large-scale gene expression analysis of ∼12,000 human genes in nine severely atherosclerotic and six non-atherosclerotic human coronary arteries using oligonucleotide microarrays. Fifty-six genes showed differential expression in atherosclerotic coronary artery tissues; expression of 55 genes was increased in atherosclerotic coronary arteries, whereas only one gene, GST, encoding a reducing agent, showed downregulated expression. The expression data of selected genes were validated by quantitative RT-PCR analysis as well as immunostaining. The associations of 49 genes with CAD appear to be novel, and they include genes encoding ICAM-2, PIM-2, ECGF1, fusin, B cell activator (BL34, GOS8), Rho GTPase activating protein-4, retinoic acid receptor responder, β2-arrestin, membrane aminopeptidase, cathepsins K and H, MIR-7, TNF-α-induced protein 2 (B94), and flavocytochrome 558. In conclusion, we have identified 56 genes whose expression is associated with CAD, and 49 of them may represent new genes linked to CAD.
KW - Array
KW - Atherosclerosis
KW - Coronary artery disease/myocardial infarction
KW - Gene expression
KW - Sudden death
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U2 - 10.1152/physiolgenomics.00181.2002
DO - 10.1152/physiolgenomics.00181.2002
M3 - Article
C2 - 12902549
AN - SCOPUS:0346502718
SN - 1531-2267
VL - 15
SP - 65
EP - 74
JO - Physiological Genomics
JF - Physiological Genomics
ER -