@article{363eaf2bf15545cabd61e7c371d59373,
title = "Identification of mosquito bloodmeals collected in diverse habitats in Malaysian borneo using COI barcoding",
abstract = "Land cover and land use change (LCLUC) acts as a catalyst for spillover of arthropod-borne pathogens into novel hosts by shifting host and vector diversity, abundance, and distribution, ultimately reshaping host–vector interactions. Identification of bloodmeals from wild-caught mosquitoes provides insight into host utilization of particular species in particular land cover types, and hence their potential role in pathogen maintenance and spillover. Here, we collected 134 blood-engorged mosquitoes comprising 10 taxa across 9 land cover types in Sarawak, Malaysian Borneo, a region experiencing intense LCLUC and concomitant spillover of arthropod-borne pathogens. Host sources of blood were successfully identified for 116 (87%) mosquitoes using cytochrome oxidase subunit I (COI) barcoding. A diverse range of hosts were identified, including reptiles, amphibians, birds, and mammals. Sixteen engorged Aedes albopictus, a major vector of dengue virus, were collected from seven land cover types and found to feed exclusively on humans (73%) and boar (27%). Culex tritaeniohynchus (n = 2), Cx. gelidus (n = 3), and Cx. quiquefasciatus (n = 3), vectors of Japanese encephalitis virus, fed on humans and pigs in the rural built-up land cover, creating potential transmission networks between these species. Our data support the use of COI barcoding to characterize mosquito–host networks in a biodiversity hotspot.",
keywords = "Aedes, Arbovirus, Bloodmeal, Borneo, Dengue virus, Host, Land cover and land use change, Mosquito, Vector",
author = "Young, {Katherine I.} and Medwid, {Joseph T.} and Azar, {Sasha R.} and Huff, {Robert M.} and Hannah Drumm and Coffey, {Lark L.} and {Jason Pitts}, R. and Michaela Buenemann and Nikos Vasilakis and David Perera and Hanley, {Kathryn A.}",
note = "Funding Information: Funding: This research was funded by an International Collaborations in Infectious Disease Research (ICIDR) grant from the National Institutes of Health, U01 AI115577 to N.V, D.P, and K.A.H, a pilot grant by the Institute for Human Infections and Immunity to N.V, an AAAS Women{\textquoteright}s International Research Collaboration Grant to K.A.H. Financial support for H.D. was provided by the Students Training in Advanced Research (STAR) Program through NIH T35 OD010956, and a Fulbright fellowship to K.I.Y. Funding Information: This research was funded by an International Collaborations in Infectious Disease Research (ICIDR) grant from the National Institutes of Health, U01 AI115577 to N.V, D.P, and K.A.H, a pilot grant by the Institute for Human Infections and Immunity to N.V, an AAAS Women's International Research Collaboration Grant to K.A.H. Financial support for H.D. was provided by the Students Training in Advanced Research (STAR) Program through NIH T35 OD010956, and a Fulbright fellowship to K.I.Y. We thank the local communities in Sarawak. Field assistants, Anita O'oi, Miko Anak, and Haziqqah Sani, M.S. Chang for assistance identifying mosquitoes, and The Institute of Biodiversity and Environmental Conservation at UNIMAS, specifically Gabriel Tonga and Alek Tuen for their assistance creating collaborations. Publisher Copyright: {\textcopyright} 2020 by the authors.",
year = "2020",
month = jun,
doi = "10.3390/tropicalmed5020051",
language = "English (US)",
volume = "5",
journal = "Tropical Medicine and Infectious Disease",
issn = "2414-6366",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
number = "2",
}