Identification of MHC class II-restricted tumor antigens recognized by CD4+ T cells

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19 Scopus citations

Abstract

CD4+ T cells play a central role in orchestrating host immune responses against cancer as well as autoimmune and infectious diseases. Identification of major histocompatibility complex (MHC) class II-restricted helper T peptides is important for development of effective vaccines. The lack of effective methods to identify such T-cell peptides is a major hurdle in the use of antigen-specific CD4+ T cells in cancer vaccines. Here we describe a genetic targeting expression system for cloning genes encoding for MHC class II-restricted tumor antigens recognized by tumor-reactive CD4+ T cells. Helper T peptides are subsequently identified by using synthetic peptides to test their ability to stimulate CD4+ T cells.

Original languageEnglish (US)
Pages (from-to)227-235
Number of pages9
JournalMethods
Volume29
Issue number3
DOIs
StatePublished - Mar 1 2003

Keywords

  • Cancer vaccines
  • CD4+ T cells
  • cDNA library
  • Helper T peptides
  • Immunotherapy
  • Major histocompatibility complex class II
  • Targeting expression
  • Tumor antigens

ASJC Scopus subject areas

  • Molecular Biology

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